Cd45 pe cyanine 7

Manufactured by Thermo Fisher Scientific

CD45 PE-cyanine 7 is a fluorescent-labeled antibody that binds to the CD45 antigen on the surface of various immune cells. It is designed for use in flow cytometry applications to identify and quantify cell populations expressing the CD45 marker.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

CD45-PE (59 citations)

3 protocols using cd45 pe cyanine 7

Comprehensive Cell-Surface Staining of Genetically Modified Bone Marrow Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

For cell-surface staining of sgRNA-introduced BM cells, antibodies against Notch1, conjugated to phycoerythrin (Notch1 PE; catalog no. 130607; BioLegend), Notch2 PE (catalog no. 130707), Notch3 PE (catalog no. 130507), CD45 PE-cyanine 7 (catalog no. 25-0451-82; eBioscience), c-Kit allophycocyanin (catalog no. 17-1171-82), CD25 allophycocyanin-e780 (catalog no. 47-0251-82), human-NGFR PE (catalog no. 12-9400-42), and a biotin-conjugated lineage cocktail [CD8α (catalog no. 13-0081-86), CD11b (catalog no. 13-0112-86), CD11c (catalog no. 13-0114-85), Gr-1 (catalog no. 13-5931-86), TER-119 (catalog no. 13-5921-85), NK1.1 (catalog no. 13-5941-85), CD19 (catalog no. 13-0193-85), TCRβ (catalog no. 13-5961-85), and TCRγδ (catalog no. 13-5711-85)] were used for staining. Prior to cell surface staining, cells were treated with 2.4G2 cell supernatant. All of the cells were analyzed using a flow cytometer, MACSQuant 10 (Miltenyi Biotec), FACSAria Fusion (BD Biosciences), or LSRFortessa (BD Biosciences), with FlowJo software (FlowJo LLC).

Romero-Wolf M., Shin B., Zhou W., Koizumi M., Rothenberg E.V, & Hosokawa H. (2020). Notch2 complements Notch1 to mediate inductive signaling that initiates early T cell development. The Journal of Cell Biology, 219(10), e202005093.

+ Open protocol

+ Expand

Flow Cytometry Analysis of T Cell Subsets

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The flow cytometry analysis was used to identify ERCs and detect the population of T cell subsets in this study. The staining process was the same as we previously mentioned [20 (link)]. Briefly, after being separated into single-cell suspension, the collected cells (100 µL/test) were stained with Zombie NIR™ (BioLegend, USA) to identify dead/live cells. And then stained these cells with fluorescent-labeled antibodies, which were purchased from BioLegend and eBioscience, including anti-human: CD45-FITC (Clone: HI30), HLA-DR-FITC (Clone: L243), CD73-FITC (Clone: AD2), CD105-PE-Cyanine 7 (Clone: SN6), and anti-mouse: CD45-PE-Cyanine7 (Clone: 30-F11), CD45-Percp-Cyanine 5.5 (Clone: 30-F11), CD3-FITC (Clone: 145-2C11), CD4-PE (Clone: GK1.5), CD4-FITC (Clone: RM4-5), CD8a-Percp-Cyanine 5.5 (Clone: 53-6.7), CD69-APC (Clone: H1.2F3), CD154-PE (Clone: SA047C3), IFN-γ-PE (Clone: XMG1.2), IL-17A-Percp-Cyanine 5.5 (Clone: eBio17B7), CD25-PE (Clone: PC61.5), Foxp3-APC (Clone: FJK-16s).

Qin H., Sun C., Kong D., Zhu Y., Shao B., Ren S., Wang H., Zhang J., Xu Y, & Wang H. (2023). CD73 mediates the therapeutic effects of endometrial regenerative cells in concanavalin A-induced hepatitis by regulating CD4+ T cells. Stem Cell Research & Therapy, 14, 277.

+ Open protocol

+ Expand

Flow Cytometric Analyses of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometric analyses were performed with Influx or Gallios (Beckman Coulter) flow cytometers. The data were analysed with FlowJo v10 software (TreeStar) or CytExpert2.3 software (Beckman Coulter). The following anti‐mouse antibodies were used: CD44‐PE (IM7), CD73‐APC (AD2), CD90‐PE (30‐H12), CD106‐Alexa Fluor 647 (429), CD45‐PE‐Cyanine7 (30‐F11), CD11b‐PE‐Cyanine7 (M1/70), PDGFRα‐APC (APA5), IL‐17A‐PE‐Cyanine7 (eBio17B7), TER‐119‐PE‐Cyanine7 (TER‐119), and IL‐22‐PE (1H8PWSR) from eBioscience; CD3‐V450 (500A2), CD8‐BV510 (53–6.7), CD31‐APC (MEC 13.3), CD34‐BV421 (RAM34), CD105‐BV421 (MJ7/18) Sca‐1‐V450 (D7), TNF‐α‐PE (MP6‐XT22) and IFN‐γ‐APC (XMG1.2) from BD; and CD4‐APCCY7 (GK1.5) from BioLegend.

Chen J., Huang J., Shi J., Li M., Zhao E., Li G., Chen X., Wang T., Li Q., Li W., Ma J., Mao W., Fang R., Hao J., Huang W., Xiang A.P, & Zhang X. (2022). Nestin+ Peyer's patch resident MSCs enhance healing of inflammatory bowel disease through IL‐22‐mediated intestinal epithelial repair. Cell Proliferation, 56(2), e13363.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!