The specific rat ELISA kits (ebioscience Co, San Diego, CA, USA) used for serum and heart homogenate TNF-α and IL-1β measurements according to the manufactory instructions.
Rat specific elisa kits
Manufactured by Thermo Fisher Scientific
Sourced in United States
Rat-specific ELISA kits are laboratory tools used for the quantitative detection and measurement of target analytes in rat biological samples. These kits utilize the Enzyme-Linked Immunosorbent Assay (ELISA) technique to provide sensitive and reliable results.
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Lab products found in correlation
5 protocols using rat specific elisa kits
1
Quantifying Inflammatory Cytokines in Rat Tissue
2
Serum and Heart IL-1β Measurement
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The specific rat ELISA kits (eBioscience Co, San Diego, CA, USA) were used for the measurement of serum and heart homogenate IL-1ßaccording to the manufacturer instructions.
3
Cytokine Quantification in Brain and Heart
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Concentrations of IL-10, IL-6, and TNFα in brain homogenates were measured using rat-specific ELISA kits per manufacturer’s protocol (Thermo Fisher Scientific, Grand Island, NY, United States). Brain homogenates were separated by centrifugation at 14,000 g for 10 min at 4°C to remove cellular debris. In addition, left ventricular myocardial concentrations of IL-6 and TNFα were analyzed by ELISA. Change in absorbance in every well was detected at 450 nm on a microplate reader. All measurements were performed in triplicate.
4
Cytokine Profiling of Cell Cultures
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After treatment followed by centrifugation at 2000 to 3000 rpm/min for 20 min, the cell culture supernatants were reserved. Following the manufacturer’s instructions, cytokine levels (IL-1β and TNF-α) were assayed using rat-specific ELISA kits (#K0223, Thermo Fisher Scientific, Waltham, MA, USA).
5
Measuring Inflammatory Cytokines in Rat Samples
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The concentrations of TNF-α and IL-6 in cell media, plasma, and brain homogenates were measured using rat-specific ELISA kits per manufacturer’s protocol (Thermo Fisher Scientific, Grand Island, NY, USA). The plasma was obtained by centrifugation at 2,000 g for 10 min at 4°C, and stored at −80°C until use. Brain homogenates were separated by centrifugation at 14,000 g for 10 min at 4°C to remove cellular debris. Change in absorbance in every well was detected at 450 nm on a microplate reader. All measurements were performed in triplicate.
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