HER2-positive BT474 human breast cancer cells were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA) and subcultured in Hybri-Care medium (ATCC) with 10% FBS at 37°C in a humidified incubator, and passaged every 4–5 days. The HER2-negative MDA-MB-468 cell line was obtained from ATCC and used as a control.4 (link)
Mda mb 468 cell line
Manufactured by American Type Culture Collection
Sourced in United States
The MDA-MB-468 cell line is a human breast adenocarcinoma cell line derived from the pleural effusion of a female patient. This cell line is commonly used in cancer research as a model for triple-negative breast cancer.
Automatically generated - may contain errors
Lab products found in correlation
Hybri care medium, by American Type Culture Collection (1 mentions)
Bt474 human breast cancer cells, by American Type Culture Collection (1 mentions)
Mda mb 231 cell line, by American Type Culture Collection (1 mentions)
Tryptone, by BD (1 mentions)
Ammonium chloride, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Yeast extract, by BD (1 mentions)
Fluorouracil 5 fu, by Merck Group (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
L glutamine penicillin streptomycin solution, by Merck Group (1 mentions)
Alexa fluor 488 nhs ester, by Thermo Fisher Scientific (1 mentions)
Biuret reagent, by Merck Group (1 mentions)
O phtalaldehyde, by Merck Group (1 mentions)
Imidazole, by Chem-Impex (1 mentions)
T cell all jk cell line, by American Type Culture Collection (1 mentions)
5 protocols using mda mb 468 cell line
1
Cultivation of Human Breast Cancer Cells
2
Antibody Preparation and Cell Lines
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All chemicals were of analytical grade and purchased from Sigma-Aldrich (St. Louis, MO) unless otherwise noted. Anti-TPP antiserum was prepared as previously described [30 (link)]. The MDA-MB-231 cell line was a generous gift from Dr. Danny Welch, and was originally obtained from ATCC. The MDA-MB-468 cell line was obtained from ATCC.
3
Biomolecular Protocols for Cell-Based Assays
4
Culturing MDA-MB-468 Breast Cancer Cells
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The MDA-MB-468 cell line was obtained from American Type Culture Collection (Manassas, VA, USA) and was cultured in RPMI-1640 supplemented with 10％ FBS, penicillin (100 U mL -1 ) , and streptomycin (100 mg mL -1 ) at 37℃ in a humidified atmosphere of 5％ CO 2 . All cells were cultured in a humidified incubator containing 5％ CO 2 at 37℃.
5
Cultivation and Treatment of T-cell ALL and Breast Cancer Cell Lines
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T-cell ALL JK cell line and MDA-MB-468 cell line, a human epithelial breast cancer cell line, were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Cells were maintained in RPMI1640 media for JK and Dulbecco’s modified Eagle medium (DMEM) (UFC-Biotech, Riyadh, Saudi Arabia) for MDA-MB-468 supplemented with 15% (v/v) Fetal bovine serum (FBS), penicillin (100 IU/mL), and streptomycin (100 µg/mL). All cell lines were maintained in a humidified incubator containing 5% CO₂ at 37°C. For all treatments, a 10-mM solution of TQ (Sigma-Aldrich, Louis, MO, USA) was prepared in 10% dimethyl sulfoxide (DMSO) (Millipore, Molsheim, France) and appropriate working concentrations were prepared with cell culture medium. The final concentration of DMSO was always less than 0.1% in both control and treated conditions.
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