Real-time PCR analysis was performed with primers and probe sets from Applied Biosystems, as described (Liu et al., 2009 (link)). Immunoblots were performed as described previously (Liu et al., 2010 (link)), using the primary antibodies anti-CD115, anti-phosphorylated (T37/46) 4E-BP1 (236B4), anti-phosphorylated (S235/236) S6 (DX57.2.2E), anti-phosphorylated ERK1/2 (D13.14.4E) , anti-phosphorylated (S473) AKT (D9E), anti-S6 (5G10), anti-4E-BP-1 (53H11), anti-ERK1/2 (137F5), anti Akt (polyclonal; all from Cell Signaling Technology), anti-SREBP2 (ab30682; Abcam), and anti-β-actin (AC-74; Sigma-Aldrich) and the secondary horseradish peroxidase–conjugated antibodies anti–rabbit IgG (W401B) and anti–mouse IgG (W402B; both from Promega).
Anti srebp2 ab30682
Manufactured by Abcam
Sourced in United States
Anti-SREBP2 (ab30682) is a primary antibody that targets the Sterol Regulatory Element Binding Protein 2 (SREBP2) protein. SREBP2 is a transcription factor that regulates the expression of genes involved in cholesterol biosynthesis and uptake.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti srebp2 ab30682
1
Real-Time PCR and Immunoblot Analysis
2
Lipid Metabolism Regulatory Proteins
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Anti-AMPK (#5831S), anti-phosphorylated AMPK (Thr 172) (#2535S), and anti-phosphorylated ACC (Ser 79) (#3661S) were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-ACC (#NB100–92011) primary antibody was purchased from Novus Biologicals (Littleton, CO, USA). Anti-CPT1A (#ab128568), anti-FAS (#ab22759), anti-HMGCR (#ab174830), anti-phosphorylated HMGCR (Ser 872) (#ab78275), anti-PPARα (#ab8934), anti-SREBP1 (#ab28481), and anti-SREBP2 (#ab30682) primary antibodies were purchased from Abcam, Inc. (Cambridge, MA, USA). Anti-UCP2 (#662047) primary antibody was purchased from Merck (Darmstadt, Germany). Pre-designed primers for SREBF1 (#FR1_Srebf1), SREBF2 (#FR1_Srebf2), FAS (FR1_Fas), ACACA (FR1_Acaca), HMGCR (FR1_Hmgcr), PPARA (FR1_Ppara), CPT1A (FR1_Cpt1a), UCP2 (FR1_Ucp2), or ACTIN-B (FR1_Actb) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). All chemicals were purchased from Sigma unless otherwise mentioned.
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