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Dextran alexa fluor 594

Manufactured by Thermo Fisher Scientific
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Dextran-Alexa Fluor 594 is a fluorescent dye-labeled polysaccharide compound. It is used as a tracer or label in various biological applications, such as cell and tissue imaging.

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Lab products found in correlation

Te2000 e, by Nikon (1 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions) Ez link sulfo nhs ss biotin, by Thermo Fisher Scientific (1 mentions) Anti rab11a, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Alexa Fluor 594 (2045 citations) Alexa 594 (538 citations) Alexa Fluors (21 citations) Dextrans (2 citations) Alexa Fluor 594-conjugated dextran (2 citations)

2 protocols using dextran alexa fluor 594

1

Cellular Uptake of Dextran-Alexa Fluor 594

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The dextran-Alexa Fluor 594 (10,000 MW) tracer was purchased from Thermo Fisher Scientific. To evaluate the cellular uptake of the tracer, the cells were washed twice with phenol red-free DMEM containing 10% fetal bovine serum and then incubated with the tracer (0.5 mg/mL) in the same medium for 8 h (Overmeyer et al. 2008 (link)). The cells were washed twice with the same medium without the tracer, and then images of live cells were acquired by laser-scanning confocal microscopy (TE2000-E, Nikon, Tokyo, Japan).
Jeon D., Kim S.W, & Kim H.S. (2019). Platycodin D, a bioactive component of Platycodon grandiflorum, induces cancer cell death associated with extreme vacuolation. Animal Cells and Systems, 23(2), 118-127.
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2

Characterizing MHC-II Antigen Presentation Dynamics

Cited 1 time
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The anti-mouse pMHC-II mAb Y3P (29 (link)) was used as a tissue culture supernatant (cells obtained by the American Type Tissue Collection, Rockville, MD) and the anti-I-Ak β-chain rabbit serum has been described previously (30 (link)). Mouse mAb anti-Rab11a was from Santa Cruz, rabbit polyclonal anti-Rab11a mAb was from Abcam, and rat-anti-mouse I-A/I-E mAb M5/114 was from BD Biosciences. Biotinylated LAMP-1 mAb 1D4B was obtained from Invitrogen (Carlsbad, CA). mAb Y810C2 was generously provided by Dr. Jon Yewdell (NIAID, NIH).
Venus-Rab11 in the lentivirus vector pLESIP was the generous gift of Dr. Roberto Weigert (NCI, NIH) and has been described previously (31 (link)). HRP-conjugated reagents were obtained from Southern Biotech. EZ-Link Sulfo-NHS-SS-Biotin, Lysotracker Red DND-99 and dextran Alexa-fluor 594 were obtained from Thermo Scientific. Reduced glutathione was obtained from Calbiochem.
Cho K.J., Ishido S., Eisenlohr L.C, & Roche P.A. (2020). Activation of Dendritic Cells Alters the Mechanism of MHC Class II Antigen Presentation to CD4 T Cells. Journal of immunology (Baltimore, Md. : 1950), 204(6), 1621-1629.
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