Whole cell lysates were obtained by using lysis buffer (50 mM Tris-HCl [pH 7.4], 150 mM NaCl, 1% Triton X-100) with a protease inhibitor cocktail (Complete Mini; Roche Applied Science, Mannheim, Germany). After electrophoresis on 6–15% SDS polyacrylamide gels, proteins were transferred onto polyvinylidene difluoride membranes (Hybond-P; GE Health Care, Buckinghamshire, UK). After blocking with Blocking-One reagent (Nacalai Tesque, Kyoto, Japan) at room temperature for 30 min, membranes were incubated with the primary and secondary antibodies. The primary antibodies used were: rabbit anti-RFP (TurboFP635) polyclonal antibody (pAb) (Thermo Fisher Scientific, Fremont, CA, USA), mouse anti–alpha-smooth muscle actin (α-SMA) monoclonal antibody (mAb) (Abcam, Cambridgeshire, UK), rabbit anti-CDH1 mAb, rabbit anti-cytokeratin 20 (CK20) mAb (Cell Signaling Technology, Danvers, MA, USA), and mouse anti–β-actin mAb (Sigma-Aldrich). The secondary antibodies used were: horseradish peroxidase–conjugated antibodies against rabbit IgG or mouse IgG (GE Healthcare). Immunoreactive bands were detected using chemiluminescence substrate (ECL Plus; GE Healthcare).
Horseradish peroxidase conjugated antibodies against rabbit igg or mouse igg
Manufactured by GE Healthcare
Horseradish peroxidase-conjugated antibodies against rabbit IgG or mouse IgG are laboratory reagents used for detection and quantification purposes. These conjugated antibodies bind to the target IgG molecules, enabling visualization or quantification through the horseradish peroxidase enzyme activity.
Automatically generated - may contain errors
Lab products found in correlation
Hybond p, by GE Healthcare (3 mentions)
Hybond, by Cytiva (3 mentions)
Mouse anti β actin mab, by Merck Group (2 mentions)
Ecl plus, by GE Healthcare (2 mentions)
Complete mini, by Roche (1 mentions)
Blocking one reagent, by Nacalai Tesque (1 mentions)
Amersham ecl chemiluminescence system, by GE Healthcare (1 mentions)
α sma, by Abcam (1 mentions)
E cadherin, by Cell Signaling Technology (1 mentions)
β actin, by Merck Group (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
Amersham ecl, by Cytiva (1 mentions)
Rabbit anti erk1 2 mab, by Cell Signaling Technology (1 mentions)
3 protocols using horseradish peroxidase conjugated antibodies against rabbit igg or mouse igg
1
Western Blot Analysis of Cell Lysates
2
Immunoblotting Analysis of Cellular Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were washed with cold phosphate-buffered saline (PBS) and lysed with the SDS buffer. Whole-cell lysates were loaded into each lane of an 8% SDS-polyacrylamide gel and electrophoretically transferred to polyvinylidene difluoride membranes (Hybond-P; GE Health Care, Buckinghamshire, UK). Membranes were incubated with primary antibodies against CD80 (EPR1157; Abcam, Cambridgeshire, UK), CD68 (EPR1392Y; Abcam), CD204 (MSR-A; TransGenic), E-cadherin (Cell Signaling Technology, Danvers, MA, USA), vimentin (Cell Signaling Technology), α-SMA (Abcam), and β-actin (Sigma-Aldrich) overnight at 4 °C. The secondary antibodies used were horseradish peroxidase-conjugated antibodies against rabbit IgG or mouse IgG (GE Healthcare). The blots were visualized using an Amersham ECL chemiluminescence system (GE Healthcare) according to the manufacturer’s protocol.
3
Monitoring Virus-Induced Cellular Changes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded in a 100-mm dish at a density of 105 cells/dish 24 h before virus infection. Cells were infected with OBP-301 or OBP-702 at the indicated MOI for 72 h. Whole-cell lysates were prepared at the indicated time points. Proteins were electrophoresed on 6%–15% SDS polyacrylamide gels and then transferred onto polyvinylidene difluoride membranes (Hybond-P; GE Healthcare, Buckinghamshire, UK). Primary antibodies used were as follows: mouse anti-p62 monoclonal antibody (mAb) (Medical & Biological Laboratories, Nagoya, Japan), mouse anti-p53 mAb (Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-Ad5 E1A mAb (BD Pharmingen, Franklin Lakes, NJ, USA), rabbit anti-KRAS mAb (Abcam, Cambridge, MA, USA), rabbit anti-PARP polyclonal antibody (pAb), rabbit anti-ERK1/2 mAb, rabbit anti-phospho-ERK1/2 mAb (Cell Signaling Technology, Danvers, MA, USA), and mouse anti-β-actin mAb (Sigma-Aldrich). Secondary antibodies used were as follows: horseradish peroxidase-conjugated antibodies against rabbit IgG or mouse IgG (GE Healthcare). Immunoreactive bands on the blots were visualized using enhanced chemiluminescence substrates (ECL Plus; GE Healthcare).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!