Irdye 800cw conjugated goat anti rabbit antibody

Cells were seeded in 6 well plates (Costar; 250000 HepG2 cells/well and 125000 A549 cells/well) 1 day before incubation with macrophages. After the incubation, proteins were extracted and PARP-1 and caspase-3 protein abundance was assessed by western blotting as described previously [13 (link)]. Primary antibodies are rabbit anti-caspase-3 antibody (Cell Signaling, #9662) and mouse anti-PARP1 antibody (BD Pharmingen, #551025). Primary antibodies mouse anti-β-actin (Sigma, #A5441) or mouse anti-α-tubulin (Sigma, # T5168) were used for normalization. IRDye 800CW-conjugated goat anti-rabbit antibody (H + L; Licor, #926-32211), IRDye 800CW-conjugated goat anti-mouse antibody (H + L; Licor, #926-32210) and IRDye 680LT-conjugated goat anti-mouse antibody (H + L; Licor, # 926–68020) were used as secondary antibodies. Quantitative analysis of fluorescence intensity was measured using the Odyssey Classic Infrared Imaging System (Licor).

Mouse monoclonal antibody 9E10 anti-HCV NS5A was kindly provided by Charles Rice^{38 (link)} and diluted 1:1000 in TCID₅₀ assays. Commercial antibodies used in Western blotting, along with their respective dilutions, are listed as follow. Mouse monoclonal antibody anti-HCV NS3 was purchased from Abcam (ab65407, 1:3,000). Mouse monoclonal antibody against GAPDH, rabbit polyclonal antibody against CRBN, and rabbit polyclonal antibody against SOD1 were respectively purchased from GeneTex (GTX28245, 1:5,000), Novus Biologicals (NBP1-91810, 1:500), and Sigma-Aldrich (HPA00140-1; 1:500). Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG and anti-rabbit IgG antibodies were obtained from Bio-Rad Laboratories (170-6516 and 170-6515, respectively, 1:3,000). IRDye® 800CW-conjugated goat anti-rabbit antibody was purchased from LI-COR (926-32211, 1:10,000).