Human placental choriocarcinoma cells (JEG-3) and normal human pulmonary epithelial cells (L-132) were purchased from the Korean Cell Line Bank (KCLB). L-132 cells were used as a control in migration assay [31] (link). Three types of genetically engineered human neural stem cells (hNSCs; HB1.F3, HB1.F3.CD , and HB1.F3.CD .IFN-β) were provided by Dr. Seung U. Kim (University of British Columbia) and used in this study. All cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM; Hyclone Laboratories) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; Hyclone Laboratories), 10 U/ml penicillin and 100 μg/ml streptomycin (Cellgro Mediatech), 10 mM HEPES (Invitrogen Life Technologies), and plasmocin as antimycoplasmal agents (Invivogene) at 37°C under 5% CO2 and 95% air in a humidified cell incubator. The cells were trypsinized with 0.05% trypsin 0.02% EDTA (PAA Laboratories).
L 132
Manufactured by Korean Cell Line Bank
The L-132 is a specialized laboratory equipment designed for cell culture applications. It provides a controlled environment for the growth and maintenance of cell lines. The core function of the L-132 is to support and sustain the optimal conditions required for cell proliferation and viability.
Automatically generated - may contain errors
Lab products found in correlation
Hepes, by Thermo Fisher Scientific (1 mentions)
Jeg 3, by Korean Cell Line Bank (1 mentions)
Fetal bovine serum (fbs), by Cytiva (1 mentions)
Dulbecco s modified eagle s medium dmem, by Cytiva (1 mentions)
Trypsin 0.02 edta, by GE Healthcare (1 mentions)
Mrc 5, by Korean Cell Line Bank (1 mentions)
Mycoplasma pcr detection kit, by iNtRON Biotechnology (1 mentions)
Human umbilical vein endothelial cells (huvec), by American Type Culture Collection (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
2 protocols using l 132
1
Culturing Human Cell Lines for Migration Assays
2
Cell Line Cultivation and Verification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The human normal prostate (RWPE-1), normal lung (L132, MRC-5), prostate cancer (PC-3), and lung cancer (H292) cell lines were obtained from the Korean Cell Line Bank (Seoul, Korea). The human lung cancer (A549), embryonic kidney (HEK293), and umbilical vein endothelial (HUVEC) cell lines were obtained from the American Type Culture Collection (Manassas, VA). Every 6 months, all cell lines were tested for mycoplasma contamination using the mycoplasma PCR detection kit (Intron Biotechnology, Sungnam, Korea). Cells were grown in medium (K-SFM for RWPE-1; DMEM for L132, MRC-5, and HEK293; RPMI-1640 for A549, PC-3, and H292 cells) supplemented with 10% FBS and antibiotics-antimycotics (100 units/mL penicillin G sodium, 100 mg/ml streptomycin, and 250 ng/ml amphotericin B [PSF]). HUVEC cells were grown in M199 medium with 20% HI-FBS, 20 μg/ml ECGS, and 50 μg/mL heparin. All cells were cultured at 37°C and 5% CO2 in a humidified atmosphere.
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