Non-labeled oligonucleotides were annealed at 10 μM concentration and diluted to 2.5 μM before data collection. All UV-Vis experiments were performed on a Cary 300 Bio UV/Vis spectrophotometer equipped with a Peltier temperature controller (Agilent Technology, Santa Clara, CA, USA). For thermal difference spectroscopy (TDS), a first spectrum was collected at 25 °C, samples were heated to 95 °C, and a second spectrum was collected. TDS was calculated by subtracting the 25 °C spectrum from the 95 °C spectrum and normalizing the maximum peak to an absorbance of 1 and the absorbance at 330 nm to 0. For thermal denaturation experiments, the absorbance at 295 nm was monitored in the temperature range from 25 to 95 °C at a heating rate of 0.5 °C/min. Data were normalized to a maximum of 1.
Peltier temperature controller
Manufactured by Agilent Technologies
Sourced in United States
The Peltier temperature controller is a precision instrument used to precisely regulate and maintain the temperature of a sample or device. It utilizes the Peltier effect, a thermoelectric phenomenon, to actively cool or heat a target area. The controller provides accurate temperature monitoring and adjustment capabilities to support various laboratory and research applications.
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Lab products found in correlation
4 protocols using peltier temperature controller
1
Thermal Characterization of Oligonucleotides
2
UV-Vis Characterization of Aqueous CD
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UV-Vis absorbance spectra of the aqueous CD dispersions were collected using a Cary 5000 Series UV-Vis-NIR Spectrophotometer from Agilent Technologies. The scans were acquired over a range of 200–800 nm in a 1-cm quartz cuvette at a speed of 600 nm/s, 1 nm resolution and a 2-nm bandwidth. A Peltier Temperature Controller from Agilent Technologies was used to heat and cool the sample cell for temperature experiments. Data collection and processing were performed using the Agilent Cary Eclipse Scan software package.
3
UV Absorption Spectra of ALA with CA
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The UV absorption
spectra of ALA (15.0 μM) without and with CA at concentrations
of 0, 30, 45, and 60 M were measured on a Cary 60 spectrophotometer
equipped with a Peltier temperature controller (Agilent Technologies,
Inc., Santa Clara, CA, USA). The protein spectra with and without
CA were recorded from 250 to 400 nm. The absorption spectra of various
concentrations of CA at pH 7.4 were also scanned. The blank was subtracted.
spectra of ALA (15.0 μM) without and with CA at concentrations
of 0, 30, 45, and 60 M were measured on a Cary 60 spectrophotometer
equipped with a Peltier temperature controller (Agilent Technologies,
Inc., Santa Clara, CA, USA). The protein spectra with and without
CA were recorded from 250 to 400 nm. The absorption spectra of various
concentrations of CA at pH 7.4 were also scanned. The blank was subtracted.
4
Enzymatic Activity Assay of Free and Immobilized Lipase
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The enzymatic activity of free and immobilized TLL was assayed using p-nitrophenyl butyrate (p-NPB) as the substrate. Briefly, 25 μg ZnOFe-TLL or a suitable amount of soluble TLL was added to 1 mL phosphate buffer (0.025 M, pH 7.5). The reaction was initiated with the addition of 0.5 mM p-NPB (diluted in DMSO). p-NP release was monitored at 405 nm using a UV–Vis spectrophotometer equipped with a Peltier temperature controller that maintained a constant temperature of 40 °C (Agilent, Santa Clara, CA, USA). Absorption values were taken every minute for a total time of ten minutes, and p-NP concentration was calculated based on a standard curve. Regarding this work, one unit of enzymatic activity (U) was defined as the amount of lipase that liberates 1 μmol of p-NP per minute per mL of reaction at 40 °C. Blank samples without enzymes were also prepared and no product was detected.
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