12 mm polyester transwells

Manufactured by Corning

Sourced in United States

The 12-mm polyester transwells are a laboratory equipment product used for cell culture experiments. They provide a physical barrier between two compartments, enabling the study of cell migration, permeability, and interaction between different cell types or between cells and their environment.

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Lab products found in correlation

Evom2 epithelial voltage meter, by World Precision Instruments (1 mentions)

Close spelling variants of this product

12-mm Transwell (2 citations)

2 protocols using 12 mm polyester transwells

Modeling EFEMP1 Mutation in iPSC-RPE

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Gene edited ARPE-19 cells EFEMP1^WT/WT and EFEMP1^KI/KI (carrying 2 copies of the mutation C > T c.1034 in the EFEMP1 gene) were seeded on 12-mm polyester transwells (Corning, NY, USA) at a density of 4 × 10⁴ cells per transwell in DMEM:F12 + 10% FBS. FBS was removed after 72 h. After 4 weeks, transwells were decellularized by incubation with sterile 0.5% Triton X-100 + 20mM NH₄OH in PBS during 5 min at 37 °C as previously described and washed with PBS thoroughly [16 (link)]. The iPSC-RPE cells were seeded immediately on the exposed ECM.

Chinchilla B, & Fernandez-Godino R. (2021). AMD-Like Substrate Causes Epithelial Mesenchymal Transition in iPSC-Derived Retinal Pigment Epithelial Cells Wild Type but Not C3-Knockout. International Journal of Molecular Sciences, 22(15), 8183.

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Measuring Epithelial Barrier Function

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The TEER assay was performed as described previously.³⁸ Caco-2/BBE cells were cultured on 24-well 12 mm polyester Transwells (Corning, Corning, NY) for 21 days. IL-6 (10 ng/ml) was added to both the upper and lower chambers of the transwell cultures. TEER was measured before and after treatment for 24 hours ± UNC0638 (500 nM) or BRD4770 (10 μM) or vehicle using an EVOM2 epithelial voltage meter (World Precision Instruments, Sarasota, FL). TEER values were calculated after subtraction of the intrinsic resistance of the cell-free filter. To measure dextran permeability, 4 kDa fluoroscein isothiocyanate (FITC)-dextran (FD4; 3 mg/ml) was added to the upper chamber without medium change. Aliquots were withdrawn from the lower chambers after 4 h and assayed for fluorescence at 515 nm with excitation at 492 nm.

Wiley J.W., Zong Y., Zheng G., Zhu S, & Hong S. (2020). Histone H3K9 methylation regulates chronic stress and IL-6-induced colon epithelial permeability and visceral pain. Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 32(12), e13941.

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