Goat anti-mouse immunoglobulins, conjugated to horseradish peroxidase (Sigma, A3673); used for western blotting in a 1:10,000 dilution. Chicken anti-mouse immunoglobulins conjugated to Alexa488 (Invitrogen; #A21200), used for IF microscopy in a 1:10,000 dilution. Donkey anti-rabbit immunoglobulins conjugated to Alexa568 (Invitrogen; #A10042), used for IF microscopy in a 1:10,000 dilution. Donkey anti-mouse immunoglobulins conjugated to Alexa568 (Invitrogen; #A10037), used for IF microscopy in a 1:10,000 dilution. Chicken anti-rabbit immunoglobulins conjugated to Alexa488 (Invitrogen; #A21441), used for IF microscopy in a 1:10,000 dilution. Monovalent Fab fragments rabbit anti-mouse (unconjugated; Fab rabbit anti-mouse IgG (H&L); Rockland Immunochemicals, #810-4102 via Biomol GmbH, Hamburg, Germany), used for IF microscopy in a 1:50 dilution.
Fab rabbit anti mouse igg h l
Manufactured by Rockland Immunochemicals
Sourced in Germany
Fab rabbit anti-mouse IgG (H&L) is a laboratory reagent used to detect and quantify mouse immunoglobulin (IgG) in various immunoassays and research applications. It consists of the antigen-binding fragment (Fab) of rabbit-derived antibodies that specifically recognize the heavy and light chains of mouse IgG.
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2 protocols using fab rabbit anti mouse igg h l
1
Immunoglobulin Detection Reagents
2
Immunofluorescence and Western Blot Antibodies
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Secondary antibodies for immunofluorescence microscopy: Chicken anti‐mouse immunoglobulins conjugated to Alexa488 (Invitrogen, Carlsbad, CA, USA, #A21200). Donkey anti‐mouse immunoglobulins conjugated to Alexa568 (Invitrogen, #A10037). Donkey anti‐rabbit immunoglobulins conjugated to Alexa568 (Invitrogen, #A10042). Chicken anti‐rabbit immunoglobulins conjugated to Alexa488 (Invitrogen, #A21441). Goat anti‐mouse immunoglobulins conjugated to Alexa647 (Invitrogen, #A21236). Monovalent Fab fragments rabbit anti‐mouse [unconjugated; Fab rabbit anti‐mouse IgG (H&L); Rockland Immunochemicals, #810‐4102 via Biomol GmbH, Hamburg, Germany], used for immunofluorescence microscopy in a 1:50 dilution. Chicken anti‐goat immunoglobulins conjugated to Alexa594 (Invitrogen, #A21468). Secondary antibodies for Western blot analyses: goat anti‐rabbit immunoglobulins conjugated to peroxidase (Sigma, #A6154), used in a 1:5,000 dilution for Western blot analyses; goat anti‐mouse immunoglobulins conjugated to peroxidase (Sigma, #A3673), used in a 1:5,000 dilution for Western blot analyses.
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