Mito id red

Manufactured by Enzo Life Sciences

MITO-ID Red is a fluorescent probe that selectively stains mitochondria in live cells. It is a cationic dye that accumulates in the mitochondrial matrix in proportion to the membrane potential.

Automatically generated - may contain errors

Lab products found in correlation

Golgi id green, by Enzo Life Sciences (2 mentions) Er id red, by Enzo Life Sciences (2 mentions) Mitosox red, by Thermo Fisher Scientific (1 mentions) Lyso id red, by Enzo Life Sciences (1 mentions) Fixation medium a, by Thermo Fisher Scientific (1 mentions) Mitotracker deep red, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

MITO-ID Red Detection Kit

4 protocols using mito id red

Mitochondrial Function in Adipose SVF Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

eWAT SVF cells were isolated from male WT or MitoFat mice age 10-12 weeks old, as described above. Cells from 2 eWAT pads per mouse were resuspended in 400 μL Wash Media and divided into 4 equal aliquots. To the aliquots were resuspended in 200 μL Wash Media (unstained control) or 200 μL Wash Media containing MitoID-Red (1:10,000 dilution; Enzo Life Sciences), CMX Red Rosamine (100 nM; ThermoFisher), or MitoSOX Red (5 μM; ThermoFisher) per manufacturer protocols. Cells were incubated at 37°C with 5% CO₂ for 15 min and then washed with 200 μL Wash Media twice and 200 μL PBS before staining for flow cytometric analysis, as described above.

Brestoff J.R., Wilen C.B., Moley J.R., Li Y., Zou W., Malvin N.P., Rowen M.N., Saunders B.T., Ma H., Mack M.R., Hykes BL J.r., Balce D.R., Orvedahl A., Williams J.W., Rohatgi N., Wang X., McAllaster M.R., Handley S.A., Kim B.S., Doench J.G., Zinselmeyer B.H., Diamond M.S., Virgin H.W., Gelman A.E, & Teitelbaum S.L. (2020). Intercellular mitochondria transfer to macrophages regulates white adipose tissue homeostasis and is impaired in obesity. Cell metabolism, 33(2), 270-282.e8.

+ Open protocol

+ Expand

Antibody Panel for Vesicular Trafficking

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies to caveolin-1 (D46G3), clathrin HC (D3C6), Rab5 (C8B1), APPLI (D83H4), EEA1 (C45B10), Rab9A (D52G8), Rab11 (D4F5) and syntaxin-6 (C34B2) rabbit antibodies were from Cell Signaling Technology. MITO-ID Red, Golgi ID Green, ER ID Red and Lyso ID Red were purchased from Enzo Life Sciences. Anti-α3 integrin (CD49c) and APC-anti-α3 integrin (CD49c) antibody (clone ASC-1), anti-CD9 antibody and FITC anti-CD9 antibody (clone H19a), PE anti-human IGF-1R (clone 1H7/CD221), PE anti-human CD115 (clone 9-4D2-1E4), PE anti-human EGFR (AY13), anti-CD59 antibody and PE anti-CD59 antibody (p282), anti-CD276 antibody and APC anti-CD276 antibody (MIH42), anti-CCR2 antibody (K036C2) and anti-CXCR4 antibody (12G5) were from Biolegend. Anti-CXCR2 antibody and APC anti-CXCR2 (Clone 6C6) were from BD Biosciences. Anti-TIMP3 antibody was from Biorbyt Ltd. (Cambridge, United Kingdom). Alexa 555 goat anti-IgG H+L antibodies were from Life Technologies. Alexa 647 anti-P2Y11 (505214) and Alexa 647 anti-MRGX2 (477533) were from R&D Systems. Anti-human LL-37 antibody (1C12) was from Hycult Biotch. Goat anti-mouse IgG (H&L):15 nm Gold was from BBI Solutions. LL-37 was synthesized by the standard FMOC chemistry, purified by reversed phase HPLC, and the mass verified by nanospray mass spectrometry. The concentration was determined by amino acid analysis (Zhang et al., 2008 (link)).

Zhang Z., Le K., La Placa D., Armstrong B., Miller M.M, & Shively J.E. (2019). CXCR2 specific endocytosis of immunomodulatory peptide LL-37 in human monocytes and formation of LL-37 positive large vesicles in differentiated monoosteophils. Bone Reports, 12, 100237.

+ Open protocol

+ Expand

Mitochondrial Imaging of Cryosectioned Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fresh tissue samples were washed with PBS and fixed in formaldehyde fixation buffer. After 48 h fixation, each tissue was merged in optimal cutting temperature compound, freeze at −80 °C in the dark, and cut into 10 μm thick tissue sections using a cryostat. The frozen tissue slides were incubated with 100 μL of diluted (1 μL MITO-ID Red in 10 mL 1× assay buffer, Enzo life sciences) reagent for 30 min and DAPI (200 nM) for additional 15 min. Finally, slides were washed three times by PBS and imaged. Images were captured by Zeiss confocal microscope (LSM 810) with a 63× oil-immersion objective.

Xi J., Li M., Jing B., An M., Yu C., Pinnock C.B., Zhu Y., Lam M.T, & Liu H. (2018). Long-Circulating Amphiphilic Doxorubicin for Tumor Mitochondria-Specific Targeting. ACS applied materials & interfaces, 10(50), 43482-43492.

+ Open protocol

+ Expand

Multiparametric Analysis of Protein and Organelle Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols

For HC and LC protein, cells (1 x 10 6 ) were fixed using a 1:1 solution of Fixation Medium A (Invitrogen) and flow cytometry buffer (PBS with 5% BSA) for 15 min at RT and then incubated with Permeabilisation Medium B (Invitrogen) containing 1:20 goat f(ab')2 antihuman IgG Alexa Fluor conjugated to 488 (Invitrogen, excitation/emission: ~500/520 nm) and goat f(ab')2 anti-human kappa conjugated to APC (Biolegend, excitation/emission: ~650/675 nm) for 15 min, before being washed and analysed. HC and LC mRNA were investigated using a PrimeFlow RNA kit with custom probes (Affymetrix, Merck) following the manufacturer protocol. Quantitation of organelles was performed by staining with Golgi-ID Green (excitation/emission: ~450/530 nm), ER-ID red (excitation/emission: ~580/660 nm), Mito-ID red (excitation/emission: ~558/690 nm; all from Enzo Life Sciences) and MitoTracker Deep Red (Invitrogen, excitation/emisssion: ~644/665 nm) using the manufacturers' protocols. DAPI (excitation/emisssion: ~340/450 nm) or Sytox nuclear (excitation/emission: ~444/480) counter-stain were used for nuclear localization.

Pekle E., Smith A., Rosignoli G., Sellick C., Smales C.M, & Pearce C. (2019). Application of Imaging Flow Cytometry for the Characterization of Intracellular Attributes in Chinese Hamster Ovary Cell Lines at the Single-Cell Level. Biotechnology journal, 14(7).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!