Analysis ls software

Manufactured by Olympus

Analysis LS software is a data analysis tool designed for use with Olympus laboratory equipment. It provides core functions for processing and analyzing data generated from Olympus instruments.

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Lab products found in correlation

Cx31 optical microscope, by Olympus (1 mentions) Bx51 optical microscope, by Olympus (1 mentions) Haematoxylin eosin h e, by Merck Group (1 mentions) Cx21 light microscope, by Olympus (1 mentions) Xylene, by Merck Group (1 mentions) Ethyl alcohol, by Merck Group (1 mentions) Bx51 light microscope, by Olympus (1 mentions)

2 protocols using analysis ls software

Histopathological Assessment of Fish Tissues

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Of the 15 fish, remaining 6 were used for histological assessment. The gill, liver and kidney tissues of the fish sampled on days 0, 7 and 14 were examined histopathologically. After fixing in Bouin’s solution for 8 h, the tissues were stored in 70% ethanol until the preparation of wax blocks. During the preparation stage, the tissues were passed through 80%, 90% and 96% absolute ethanol, respectively. Tissues were then passed through xylene and embedded in paraffin. Five µm cross-sections were cut from the paraffin blocks and stained with hematoxylin and eosin (H&E) for routine wax histology. Slides were examined under an Olympus CX31 optical microscope. Photographs were captured by a camera attached to an Olympus BX51 optical microscope using Olympus Analysis LS software.

Kaya H., Duysak M., Akbulut M., Yılmaz S., Gürkan M., Arslan Z., Demir V, & Ateş M. (2016). Effects of sub-chronic exposure to zinc nanoparticles on tissue accumulation, serum biochemistry and histopathological changes in tilapia (Oreochromis niloticus). Environmental toxicology, 32(4), 1213-1225.

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Histopathological Examination of Tadpoles

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At the end of the experiment (hour 120), all surviving tadpoles were evaluated for histopathological changes by fixing them in Bouin's solution for 8 h and then preserving them in 70 % ethyl alcohol (Sigma-Aldrich) until the preparation day. On the preparation day, the tadpoles were passed through 80 %, 90 %, 96 %, and absolute ethyl alcohol series and later through xylene (Sigma-Aldrich) and paraffin series to be embedded in paraffin blocks. We made 8-µmthick cross sections and stained them with haematoxylin & eosin (H&E; Sigma-Aldrich) for histopathological examination with an Olympus CX21 light microscope. We also took photographs with a camera adapted to the Olympus BX51 light microscope and analysed them with Olympus Analysis LS software.

Gürkan M, & Hayretdağ S. (2015). Acute toxicity of maneb in the tadpoles of common and green toad. Arhiv za higijenu rada i toksikologiju, 66(3).

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