Reagents were purchased from Sigma-Aldrich, unless stated otherwise. Stock solutions were made and diluted in ACSF just before bath application. Drugs used were A1R selective antagonist DPCPX (100 nM), A1R selective agonist N6-CPA (1 µM, Tocris Bioscience), Ecto-5′-nucleotidase/CD73 inhibitor AMP-CP (200 μM, Tocris Bioscience), GAT-3 blocker (S)-SNAP 5114 (100 μM, Tocris Bioscience), GABABR selective antagonist CGP55845A (2 μM, Tocris Bioscience), GABAAR selective antagonist Gabazine (5 μM), mGluR5 selective antagonist MPEP (25 μM, Tocris Bioscience), selective calcium chelating reagent BAPTA tetrapotassium salt (0.1 or 20 mM), NMDAR antagonist AP-5 (20 μM), AMPA/kainate receptor antagonist NBQX (10 μM, Tocris Bioscience).
Ampcp
Manufactured by Bio-Techne
Sourced in United Kingdom
AMPCP is a laboratory reagent used in biochemical and molecular biology applications. It functions as a nucleotide pyrophosphatase inhibitor. The core purpose of AMPCP is to inhibit the enzymatic activity of nucleotide pyrophosphatases, which are involved in the hydrolysis of various nucleotide phosphates.
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Lab products found in correlation
3 protocols using ampcp
1
Investigating Neuromodulatory Mechanisms
2
Pharmacological Modulation of A1 Receptors
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Experiments were conducted using the A1-R agonist CCPA (Tocris Bioscience Cat. No. 1705, Abingdon, United Kingdom) dissolved in a mixture of dimethyl sulfoxide (DMSO—Sigma Aldrich Cat. No. PHR1309) and distilled water (ratio 1:100), the A1-R antagonist PSB36 (Tocris Bioscience Cat. No. 2019, Abingdon, United Kingdom) dissolved in a mixture of DMSO and distilled water (ratio 1:100), the CD73 inhibitor AMPCP (Tocris Bioscience Cat. No. 3633, Abingdon, United Kingdom) dissolved in distilled water. DMSO (1%) was used as vehicle (Figure 1A ).
3
Thyroid Hormone Modulation in ADLP Mouse Model
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For T3 administration in ADLP mice, 3,3′,5-triiodo-l-thyronine sodium salt (T3; Sigma-Aldrich, T6397) was prepared at 50 mg/ml in 1 M NaOH and diluted in saline to obtain a stock solution of 0.3 mg/ml. Vehicle control consisted of 6 mM NaOH in saline. Twenty-week-old ADLP mice were injected intraperitoneally with 100 μl of vehicle or T3 (corresponding to 1.0 mg/kg) 5 days per week for 8 weeks. For the injection of AMPCP (Tocris, no. 3633), 20-week-old Cx3cr1gfp/+ mice received an intraperitoneal injection of 100 μl of vehicle (distilled water) or AMPCP (10 mg/kg) 5 days per week for 4 weeks. Eight-week-old ADLPAPP/PS1 mice received an intraperitoneal injection of 100 μl of vehicle (distilled water) or AMPCP (10 mg/kg) 5 days per week for 8 weeks to examine the change in amyloid pathology.
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