Oxidative DNA damage was examined using a DNA damage (8-OHdG) ELISA kit (StressMarq Biosciences, Victoria, BC, Canada) [56 (link)], following the manufacturer’s instructions. The absorbance at 450 nm was measured by Glomax Multi Detection System Spectrophotofluorimeter (Promega Corporation, Milan, Italy). The content of 8-OHdG was calculated using a calibration curve and expressed in nanograms (ng) of 8-OHdG per mL of cell lysates.
Glomax multi detection system spectrophotofluorimeter
Manufactured by Promega
Sourced in Italy
The Glomax Multi Detection System Spectrophotofluorimeter is a versatile laboratory instrument that combines spectrophotometric and fluorometric detection capabilities. It is designed to measure the absorbance and fluorescence of samples within a range of wavelengths.
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Lab products found in correlation
2 protocols using glomax multi detection system spectrophotofluorimeter
1
Quantifying Oxidative DNA Damage via ELISA
2
Antioxidant Enzyme Activity Assay
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The total activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were measured in K562 and Vero cells treated with 0.75 mg/mL of S.M. for 48 h. The tests were performed by commercially available ELISA (enzyme-linked immunosorbent assay) test kits, according to a previously reported protocol [55 (link)], and absorbances were measured spectrophotometrically (Glomax Multi detection System Spectrophotofluorimeter, Promega Corporation). Data were expressed in units (U) for milligrams (mg) of proteins.
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