Solanine

Manufactured by Merck Group

Sourced in United States

Solanine is a laboratory equipment product manufactured by Merck Group. It is a device used for the detection and measurement of solanine, a glycoalkaloid compound found in certain plants of the nightshade family. Solanine serves as a core function in analytical and research applications that require the identification and quantification of this specific compound.

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α-solanine (12 citations)

5 protocols using solanine

Quantitative Analysis of Glycoalkaloids

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Analytical standards of solanine,
chaconine, solasonine, solamargine, tomatine and their aglycons solanidine,
solasodine, and tomatidine were purchased from Sigma-Aldrich (Steinheim,
Germany), Carl Roth (Karlsruhe, Germany), and Extrasynthese (Genay,
France). The standards were solved in methanol, obtaining stock solutions
of 1 mM for storage at −18 °C, respectively.
The
solvents used for high-performance liquid chromatography coupled to
quadrupole time-of-flight mass spectrometery (HPLC–QToF–MS)
and high-performance liquid chromatography coupled to tandem mass
spectrometery (HPLC–MS/MS) analysis (HPLC–MS grade)
and all other chemicals used for incubation experiments were purchased
from Fisher Scientific (Schwerte, Germany), Merck (Darmstadt, Germany),
or Sigma-Aldrich (Steinheim, Germany) in gradient or reagent quality.
Purified water was obtained by a Purelab flex 2 system (ELGA LabWater
Veolia Water Technologies, Celle, Germany).

Kasimir M., Wolbeck A., Behrens M, & Humpf H.U. (2023). Intestinal Metabolism of Selected Steroidal Glycoalkaloids in the Pig Cecum Model. ACS Omega, 8(20), 18266-18274.

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Solanine Effects on Pancreatic Cancer

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Human pancreatic cancer cell lines, SW1990 and Panc-1 (Shanghai Institutes for Biological Sciences), were cultured in RPMI 1640 containing 10% FBS. Cell density was adjusted to 2 × 10⁴ cells per square centimeter before the addition of solanine (Sigma). Athymic nude nu/nu mice were obtained from the Shanghai Laboratory Animal Center at the Chinese Academy of Sciences, Shanghai, China. Animals were maintained at the Laboratory Animal Center of Wenzhou Medical University. The animal experiment protocol was approved by the Institutional Animal Committee of Wenzhou Medical University.

Sun H., Lv C., Yang L., Wang Y., Zhang Q., Yu S., Kong H., Wang M., Xie J., Zhang C, & Zhou M. (2014). Solanine Induces Mitochondria-Mediated Apoptosis in Human Pancreatic Cancer Cells. BioMed Research International, 2014, 805926.

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Cell Viability Assay Protocol

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Crystal violet, propidium iodide, acetylcholine, solanine, sodium dodecyl sulfate (SDS), Triton X-100, trypsin, and trypan blue were purchased from Sigma Chemical Co (St. Louis, MO). Fetal bovine serum was purchased from Life Technologies (Auckland, New Zealand). Dimethyl sulfoxide was purchased from Wako Pure Chemical Industries (Saitama, Japan). Vimentin, E-cadherin, and GAPDH antibodies were purchased from Santa Cruz (Santa Cruz, CA).

Lin L.T., Choong C.Y, & Tai C.J. (2020). Solanine Attenuates Hepatocarcinoma Migration and Invasion Induced by Acetylcholine. Integrative Cancer Therapies, 19, 1534735420909895.

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Assessing Anti-Inflammatory Properties of Natural Compounds

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The following drugs were used to execute the experimental protocols: Croton oil, hexadecyltrimethylammonium bromide (HTAB), tetramethylbenzidine (TMB), dexamethasone, rutin, gallic acid, ascorbic acid, chlorogenic acid, rosmarinic acid, solanine, DPPH, ammonium phosphate monobasic (all from Sigma, St. Louis, MO, USA), hematoxylin-eosin, acetonitrile, butanol, paraffin, ethanol, and Folin-Ciocalteu (all from Merck, Whitehouse Station, New Jersey, USA), Isoflurane (Baxter, São Paulo, Brazil), sodium acetate, acetone, acetic acid, and formaldehyde (all from Vetec, Rio de Janeiro, Brazil).

Piana M., Camponogara C., Boligon A.A, & Oliveira S.M. (2017). Solanum paranense Extracts and Solanine Present Anti-Inflammatory Activity in an Acute Skin Inflammation Model in Mice. Evidence-based Complementary and Alternative Medicine : eCAM, 2017, 4295680.

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Solanine Inhibition of LAB Growth

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The glycoalkaloid inhibitory effect on LAB growth [25 (link)] was evaluated by adding 200 ppm of solanine (Sigma-Aldrich, St. Louis, MO, USA) to the MRS broth further inoculated with each of the selected LAB strains (9 log₁₀ CFU/mL) and incubated at 30 °C for 72 h (in triplicate). Viable cell counts (log₁₀ CFU/mL) [29 ] were determined at 0 h and after 24 h, 48 h, and 72 h of incubation.

Pereira N., Alegria C., Aleixo C., Martins P., Gonçalves E.M, & Abreu M. (2021). Selection of Autochthonous LAB Strains of Unripe Green Tomato towards the Production of Highly Nutritious Lacto-Fermented Ingredients. Foods, 10(12), 2916.

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