acetic, propionic, butyric, isobutyric,
isovaleric, and valeric organic acids) present in
the cecal content was measured using an HPLC
system (Jasco, Tokyo, Japan) according to the
method described by Miwa et al.[17 (link)]. The
ultraviolet-visible detector (UV-2075 PLUS, Jasco)
was set to 400 nm and a YMC-Pack FA column (6 ×
250 mm; YMC Co., Ltd., Kyoto, Japan) was used with
the column oven (CO-2065 PLUS) heated to 50°C. The
mobile phase consisted of
acetonitrile:methanol:water (30:16:54, v/v; pH
4.5) at a flow rate of 1.2 mL/min. Organic acids
were labeled using a Short and Long-Chain Fatty
Acid Analysis Kit (YMC) according to the
manufacturer’s instructions.