RIP buffer (Millipore, Billerica, MA, USA) was used to lyse CRC cells, and the cell extract was incubated with anti-Argonaute-2 (anti-Ago2; Millipore) or anti-Immunoglobulin G (anti-IgG; Millipore) beads. The immunoprecipitated RNAs were identified by qRT-PCR.
Anti immunoglobulin g anti igg
Manufactured by Merck Group
Sourced in United States
Anti-Immunoglobulin G (anti-IgG) is a laboratory reagent used to detect and measure the presence of IgG antibodies in biological samples. It functions by binding to IgG molecules, enabling their identification and quantification.
Automatically generated - may contain errors
3 protocols using anti immunoglobulin g anti igg
1
Argonaute-2 Immunoprecipitation for RNA Profiling
2
RIP Assay for LINC00659 and miR-760 Interactions
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RNA immunoprecipitation (RIP) assay was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore), anti-Immunoglobulin G (anti-IgG; Millipore) and anti-Argonaute-2 (anti-ago2; Millipore). SW620 and HT29 cells after transfection for 48 h were lysed in RIP buffer (Millipore), and proteins were incubated with magnetic beads conjugated with anti-ago2, and anti-IgG served as a control. qRT-PCR was conducted to detect the abundance of LINC00659 and miR-760.
3
Confirmation of circRNA-miRNA Interaction
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RIP experiment was employed to confirm the binding relation between circ_0004104 and miR-328-3p with Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore). Cell extracts were prepared using RIP lysis buffer, and anti-Argonaute2 (anti-Ago2; Millipore) or anti-Immunoglobulin G (anti-IgG; Millipore)-pre-coated magnetic beads were incubated with cell lysates. The levels of enriched RNAs were measured by RT-qPCR.
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