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αkdm2a

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Anti-KDM2A (αKDM2A) is a primary antibody that recognizes the KDM2A protein. KDM2A is a histone demethylase that removes methyl groups from histone H3 at lysine 36 (H3K36). This antibody can be used to detect and study the expression and localization of KDM2A in various biological samples.

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Lab products found in correlation

Streptavidin beads, by New England Biolabs (2 mentions) Fulvestrant from, by MedChemExpress (2 mentions) Dynabeads protein a, by Thermo Fisher Scientific (2 mentions) α flag, by Merck Group (2 mentions) α gapdh, by Santa Cruz Biotechnology (2 mentions) Protein g, by Thermo Fisher Scientific (2 mentions) Rnase a t, by Thermo Fisher Scientific (2 mentions) αpol 2, by Santa Cruz Biotechnology (2 mentions) αrad21, by Abcam (2 mentions) αlamina c, by Abcam (2 mentions) Hispurtm cobalt superflow agarose beads, by Thermo Fisher Scientific (2 mentions) Rnase t1 cocktail, by Thermo Fisher Scientific (2 mentions) Anti ha magnetic beads, by Thermo Fisher Scientific (2 mentions) 17β estradiol e2, by Merck Group (2 mentions) Itaq universal sybr green supermix, by Bio-Rad (2 mentions)

2 protocols using αkdm2a

1

Antibodies and Reagents for Epigenetic Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in this study include: αERα (Bethyl #A300–495A) for ChIP-qPCR, ChIP-Seq, RIP-qPCR, PAR-CLIP and immunoblotting; αKDM2A (ProteinTech #24311–1-AP) for ChIP-qPCR; αPol II (Santa Cruz Biotechnology #SC-9001, #SC-56767 and Abcam #ab817) for ChIP-qPCR; αRAD21 (Abcam #ab992) for ChIP-qPCR. Other antibodies that were applied in western blot include: αGST (ProteinTech #66001–2); αHis (ProteinTech #66005–1); αHA (BioLegend, #901501); αNEDD4 (CST #3607); αPol II (Santa Cruz Biotechnology #SC-56767), αLaminA/C (Abcam, # ab8984), αGAPDH (Santa Cruz Biotechnology #sc-365062) and αFLAG (Sigma #F1804). Reagents that were utilized for immunoprecipitation purposes are: anti-HA magnetic beads (Thermo Fisher #88837); streptavidin beads (NEB #S1420S); HisPurTM cobalt Superflow agarose beads (Thermo Fisher #25228); Dynabeads Protein A (Thermo Fisher #10006D) or Protein G (Thermo Fisher #10007D). Normal anti-rabbit IgG antibody was purchased from Invitrogen (#02–6102), 17β-estradiol (E2) from Sigma (#E2758), fulvestrant from Medchem Express (#HY13636), RNase A/T from Thermo Fisher (#EN0551), RNase/T1 cocktail from Thermo Fisher (#AM2286) and iTaq Universal SYBR Green Supermix from BioRad (1725124). Antibodies information can also be found in Key Resources Table.
Yang M., Lee J.H., Zhang Z., De La Rosa R., Bi M., Tan Y., Liao Y., Hong J., Du B., Wu Y., Scheirer J., Hong T., Li W., Fei T., Hsieh C.L., Liu Z., Li W., Rosenfeld M.G, & Xu K. (2020). Enhancer RNAs Mediate Estrogen-Induced Decommissioning of Selective Enhancers by Recruiting ERα and Its Cofactor. Cell reports, 31(12), 107803.
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2

Antibodies and Reagents for Epigenetic Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in this study include: αERα (Bethyl #A300–495A) for ChIP-qPCR, ChIP-Seq, RIP-qPCR, PAR-CLIP and immunoblotting; αKDM2A (ProteinTech #24311–1-AP) for ChIP-qPCR; αPol II (Santa Cruz Biotechnology #SC-9001, #SC-56767 and Abcam #ab817) for ChIP-qPCR; αRAD21 (Abcam #ab992) for ChIP-qPCR. Other antibodies that were applied in western blot include: αGST (ProteinTech #66001–2); αHis (ProteinTech #66005–1); αHA (BioLegend, #901501); αNEDD4 (CST #3607); αPol II (Santa Cruz Biotechnology #SC-56767), αLaminA/C (Abcam, # ab8984), αGAPDH (Santa Cruz Biotechnology #sc-365062) and αFLAG (Sigma #F1804). Reagents that were utilized for immunoprecipitation purposes are: anti-HA magnetic beads (Thermo Fisher #88837); streptavidin beads (NEB #S1420S); HisPurTM cobalt Superflow agarose beads (Thermo Fisher #25228); Dynabeads Protein A (Thermo Fisher #10006D) or Protein G (Thermo Fisher #10007D). Normal anti-rabbit IgG antibody was purchased from Invitrogen (#02–6102), 17β-estradiol (E2) from Sigma (#E2758), fulvestrant from Medchem Express (#HY13636), RNase A/T from Thermo Fisher (#EN0551), RNase/T1 cocktail from Thermo Fisher (#AM2286) and iTaq Universal SYBR Green Supermix from BioRad (1725124). Antibodies information can also be found in Key Resources Table.
Yang M., Lee J.H., Zhang Z., De La Rosa R., Bi M., Tan Y., Liao Y., Hong J., Du B., Wu Y., Scheirer J., Hong T., Li W., Fei T., Hsieh C.L., Liu Z., Li W., Rosenfeld M.G, & Xu K. (2020). Enhancer RNAs Mediate Estrogen-Induced Decommissioning of Selective Enhancers by Recruiting ERα and Its Cofactor. Cell reports, 31(12), 107803.
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