Anti ikkα primary antibody

Manufactured by Cell Signaling Technology

Anti‐IKKα primary antibody is a laboratory reagent used to detect the presence and quantify the levels of IKKα (IκB kinase alpha) protein in biological samples. IKKα is a key component of the NF-κB signaling pathway, which regulates various cellular processes. This antibody can be utilized in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression and localization of IKKα in research applications.

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Lab products found in correlation

Anti β actin primary antibody, by Abcam (2 mentions) Anti rabbit igg 7074, by Cell Signaling Technology (2 mentions) Anti fabp5 primary antibody, by Abcam (2 mentions) Anti p ikkα ser176 primary antibody, by Biorbyt (2 mentions) Anti ikβ ab32518, by Abcam (2 mentions) Anti nf κb ab32360, by Abcam (2 mentions)

Spelling variants of this product

Anti-IKKα (40 citations) IKKα antibody (4 citations)

2 protocols using anti ikkα primary antibody

Western Blot Analysis of FABP5, IKK, and NF-κB Signaling

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Western blot analysis was performed as previously described¹⁸ and each western blot was repeated at least three times. Antibodies used in this study were shown as below: anti‐FABP5 primary antibody was purchased from Abcam (ab84028). Anti‐β‐actin primary antibody was purchased from Abcam and served as an internal control (ab115777). Anti‐IKKα primary antibody was purchased from Cell Signaling Technology (#11930). Anti‐p‐IKKα (Ser176) primary antibody was purchased from Biorbyt (orb544342). Anti‐IKβ (ab32518) and anti‐NF‐κB (ab32360) primary antibodies were purchased from Abcam. Anti‐Rabbit IgG (#7074) and Anti‐Mouse‐IgG (#7076) were purchased from Cell Signaling Technology and served as negative controls. The raw unedited bands of all western blot images were showed in the Figure S7.

Wang Y., Wahafu A., Wu W., Xiang J., Huo L., Ma X., Wang N., Liu H., Bai X., Xu D., Xie W., Wang M, & Wang J. (2021). FABP5 enhances malignancies of lower‐grade gliomas via canonical activation of NF‐κB signaling. Journal of Cellular and Molecular Medicine, 25(9), 4487-4500.

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Western Blot Antibody Analysis

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Western blot analysis was performed as previously described [18] . Antibodies used in this study were shown as below: anti-FABP5 primary antibody was purchased from Abcam (ab84028). Anti-β-actin primary antibody was purchased from Abcam and served as an internal control (ab115777). Anti-IKKα primary antibody was purchased from Cell Signaling Technology (#11930). Anti-p-IKKα (Ser176) primary antibody was purchased from Biorbyt (orb544342). Anti-IKβ (ab32518) and anti-NF-κB (ab32360) primary antibodies were purchased from Abcam. Anti-Rabbit IgG (#7074) and Anti-Mouse-IgG (#7076) were purchased from Cell Signaling Technology and served as negative controls.

Wang J., Alafate W., Wang Y., Wu W., Xiang J., Ma X., Wang N., Liu H., Bai X., Huo L., Xu D., Wang M., & Xie W. (2020). FABP5 enhances malignancies of lower-grade gliomas via canonical activation of NF-κB signaling.

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