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Tsc2 sc 893

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TSC2 (sc-893) is a laboratory reagent produced by Santa Cruz Biotechnology. It is a mouse monoclonal antibody that recognizes the TSC2 protein. The TSC2 protein is a component of the tuberous sclerosis complex (TSC), which is involved in the regulation of cell growth and proliferation.

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Lab products found in correlation

Paxillin, by Thermo Fisher Scientific (2 mentions) Ab16726, by Abcam (2 mentions) Ab59269, by Abcam (2 mentions) Ab28824, by Abcam (2 mentions) β actin, by Santa Cruz Biotechnology (2 mentions) Rictor, by Thermo Fisher Scientific (2 mentions) Sc 7985 r, by Santa Cruz Biotechnology (2 mentions) P akt, by Santa Cruz Biotechnology (2 mentions) Phosphorylated rb s780, by Cell Signaling Technology (2 mentions) Ptsc2, by Abcam (2 mentions) Pfkhr ser319, by Santa Cruz Biotechnology (2 mentions) Pbad ser136, by Abcam (2 mentions) Paxillin, by Merck Group (2 mentions) Cyclin d1, by Santa Cruz Biotechnology (2 mentions)

2 protocols using tsc2 sc 893

1

Antibody-based Analysis of Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibodies used in western blot analysis were cyclin D1 (sc-20044, Santa Cruz), b-actin (sc-47778, Santa Cruz), Akt1 (sc-5298, Santa Cruz), pAkt1 Ser473 (#9018, CST), pAkt1/2/3 Ser473 (sc-7985-R, Santa Cruz), TSC2 (sc-893, Santa Cruz), pTSC2 Ser939 (ab59269, Abcam), FKHR (sc-11350, Santa Cruz), pFKHR Ser319 (sc-101682, Santa Cruz), cyclin A2 (ab16726, Abcam), Bad (sc-8044, Santa Cruz), pBad Ser136 (ab28824, Abcam), Rictor (SC#271081, H11) and phosphorylated RB (S780) (Cell Signaling). Mouse anti-FLAG (M2), mouse anti-vinculin (hVIN-1) antibodies were from Sigma (St. Louis, MO). The antibody used for Immunoprecipitation was HA (sc-805, Santa Cruz). The antibodies used for immunohistochemistry and immunofluorescence were cyclin D1 (sc-20044, Santa Cruz), for Akt Ser473 phosphorylation (pAkt1/2/3 Ser473) (sc-7985-R, Santa Cruz), for pAkt1 Ser473 (#9018, CST), TSC2 (sc-893, Santa Cruz), pTSC2 Ser939 (ab59269, Abcam), pFKHR Ser319 (sc-101682, Santa Cruz), pBad Ser136 (ab28824, Abcam), cyclin A2 (ab16726, Abcam), paxillin (05–417, Millipore-Sigma), Rictor (H278, sc-99004), tyrosine phosphorylated paxillin (44–722G, Thermo Fisher), PACSIN2 (10518–2-AP, Proteintech).
Chen K., Jiao X., Di Rocco A., Shen D., Xu S., Ertel A., Yu Z., Di Sante G., Wang M., Li Z., Pestell T.G., Casimiro M.C., Skordalakes E., Achilefu S, & Pestell R.G. (2020). Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation. Cell reports, 32(11), 108151.
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2

Antibody-based Analysis of Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibodies used in western blot analysis were cyclin D1 (sc-20044, Santa Cruz), b-actin (sc-47778, Santa Cruz), Akt1 (sc-5298, Santa Cruz), pAkt1 Ser473 (#9018, CST), pAkt1/2/3 Ser473 (sc-7985-R, Santa Cruz), TSC2 (sc-893, Santa Cruz), pTSC2 Ser939 (ab59269, Abcam), FKHR (sc-11350, Santa Cruz), pFKHR Ser319 (sc-101682, Santa Cruz), cyclin A2 (ab16726, Abcam), Bad (sc-8044, Santa Cruz), pBad Ser136 (ab28824, Abcam), Rictor (SC#271081, H11) and phosphorylated RB (S780) (Cell Signaling). Mouse anti-FLAG (M2), mouse anti-vinculin (hVIN-1) antibodies were from Sigma (St. Louis, MO). The antibody used for Immunoprecipitation was HA (sc-805, Santa Cruz). The antibodies used for immunohistochemistry and immunofluorescence were cyclin D1 (sc-20044, Santa Cruz), for Akt Ser473 phosphorylation (pAkt1/2/3 Ser473) (sc-7985-R, Santa Cruz), for pAkt1 Ser473 (#9018, CST), TSC2 (sc-893, Santa Cruz), pTSC2 Ser939 (ab59269, Abcam), pFKHR Ser319 (sc-101682, Santa Cruz), pBad Ser136 (ab28824, Abcam), cyclin A2 (ab16726, Abcam), paxillin (05–417, Millipore-Sigma), Rictor (H278, sc-99004), tyrosine phosphorylated paxillin (44–722G, Thermo Fisher), PACSIN2 (10518–2-AP, Proteintech).
Chen K., Jiao X., Di Rocco A., Shen D., Xu S., Ertel A., Yu Z., Di Sante G., Wang M., Li Z., Pestell T.G., Casimiro M.C., Skordalakes E., Achilefu S, & Pestell R.G. (2020). Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation. Cell reports, 32(11), 108151.
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