Log In Sign up
The largest database of trusted experimental protocols

Mouse anti mycn

Manufactured by Santa Cruz Biotechnology
Visit Supplier
Sourced in United States

Mouse anti-MYCN is an antibody product used for the detection and analysis of MYCN protein in various experimental applications. It is a tool for researchers to study the MYCN gene and its related biological processes.

Automatically generated - may contain errors

Lab products found in correlation

Enhanced chemoluminescence, by PerkinElmer (1 mentions) Mouse anti β tubulin, by Santa Cruz Biotechnology (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Mouse anti gapdh, by Abcam (1 mentions) Anti mouse hrp, by Thermo Fisher Scientific (1 mentions)

2 protocols using mouse anti mycn

1

Quantitative Analysis of Protein Expression

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA extraction was carried out with trizol reagent (Invitrogen), and quantitative reverse transcription-PCR (Q-RT–PCR) was performed as described.34 (link)Total protein extracts were obtained and separated as described.34 (link) The following MoAbs were used: mouse anti-MYCN and mouse anti-β-tubulin (Santa Cruz Biotechnology, Santa Cruz, CA, USA); and rat anti-Gal-3 purified monoclonal antibody (Space Import & Export). Immunoreactive bands were visualized by enhanced chemoluminescence (Perkin Elmer, Waltham, MA, USA).
Veschi V., Petroni M., Bartolazzi A., Altavista P., Dominici C., Capalbo C., Boldrini R., Castellano A., McDowell H.P., Pizer B., Frati L., Screpanti I., Gulino A, & Giannini G. (2014). Galectin-3 is a marker of favorable prognosis and a biologically relevant molecule in neuroblastic tumors. Cell Death & Disease, 5(3), e1100-.
+ Open protocol
+ Expand
2

ChIP-seq and Western Blot Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibodies used for ChIP were IgG (cell signaling 2729), H3K4me1 (Abcam ab8895), H3K4me3 (Abcam ab8580), H3K9ac (Abcam ab10812), H3K27ac (Abcam ab4729), H3K27me3 (Active motif 39155), and H3K36me3 (Abcam ab9050). The following antibodies were used for Western blot: mouse anti-SPT16 (BioLegend 607002, 1:1000), mouse anti-SSRP1 (BioLegend 609702, 1:500), mouse anti-MYCN (Santa Cruz Biotechnology Sc53993, 1:1000), mouse anti-γH2AX (Abcam ab26350, 1:1000), mouse anti-GAPDH (Abcam ab8245, 1:10000), and anti-mouse HRP (Thermo Scientific 32430, 1:1500).
Hu X., Zheng W., Zhu Q., Gu L., Du Y., Han Z., Zhang X., Carter D.R., Cheung B.B., Qiu A, & Jiang C. (2019). Increase in DNA Damage by MYCN Knockdown Through Regulating Nucleosome Organization and Chromatin State in Neuroblastoma. Frontiers in Genetics, 10, 684.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!