zCDX models were developed by a CRO (BioReperia AB). Transgenic Tg(fli1:EGFP)y1 zebrafish embryos were raised at 28 °C for 48 h in E3 embryo medium (containing per liter: 0.286 g NaCl, 0.048 g CaCl2, 0.081 g MgSO4 and 0.0126 g KCl with 0.2 mM 1-Phenyl-2-Thiourea aka PTU). At 2 days post-fertilization, embryos were injected subcutaneously in the perivitelline space with transfected parental and CDDP-R cells previously labeled with FAST Dil™ oil (Thermo Fisher Scientific) and treated with ± Cisplatin 5 mg/L for 3 days. Images of tumors were taken by using a fluorescent stereoscope with a K5 camera (Leica) and LAS X software v3.7.1.21655 at 100× magnification with no binning. Images of tumors were taken right after injection (day 1) and after drug treatment (day 4). Images were automatically analyzed by using the HuginMunin software v2.7.0.0 (BioReperia AB). Tumor growth regression was calculated by dividing the number of tumor pixels on day 4 by the number of tumor pixels on day 1 in the same embryo and multiplied by 100.
Las x software v
Manufactured by Leica
LAS X software v is a digital imaging and data analysis software solution developed by Leica Microsystems. It serves as the core operating platform for Leica's microscopes and other imaging devices, providing users with tools for image acquisition, processing, and analysis.
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2 protocols using las x software v
1
Zebrafish Xenograft for Drug Response
2
Microscopy Technique for Chromosome Ideogram
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Hybridization signals were examined using a Leica DM5500B microscope equipped with a Hamamatsu ORCA-FLASH4.0 camera and controlled by Leica LAS X software v2.0. Digital images were processed using Adobe Photoshop CS5 (Adobe Systems Incorporated, USA) extended version 12.0 × 64.
The ideogram for H1, H16, H7, H. vulgare and T. aestivum chromosomes was based on the hybridization patterns of the probes used in this work and the morphology of chromosomes previously described (Cabrera et al. 1995; Pedersen and Langridge 1997; Prieto et al. 2004; Kato 2011; Szakács et al. 2013; Komuro et al. 2013; Tang et al. 2014) .
The ideogram for H1, H16, H7, H. vulgare and T. aestivum chromosomes was based on the hybridization patterns of the probes used in this work and the morphology of chromosomes previously described (Cabrera et al. 1995; Pedersen and Langridge 1997; Prieto et al. 2004; Kato 2011; Szakács et al. 2013; Komuro et al. 2013; Tang et al. 2014) .
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