The list of pharmacologically active compounds (LOPAC®) library contains 1280 experimentally validated small molecules that belong to diverse chemical classes. The ENZO® SCREEN-WELL® FDA-approved drug library V2 contains 774 FDA drug compounds belonging to various indication classes. LOPAC® and ENZO® compound libraries were kindly provided by Fraunhofer ITMP in Hamburg. Screening compounds were maintained in DMSO at 10 mM and stored on 384-well plates (Echo Qualified 384-well plates, Labcyte, San Jose, CA, USA) at −20 °C. The composition of the LOPAC® and ENZO® libraries is shown in supplementary Tables S1 and S2 , respectively.
I-BET151, Ro 11-1464, trichostatin A (TSA), and suberoylanilide hydroxamic acid (SAHA) were purchased from Sigma Aldrich (St. Louis, MO, USA). Stock solutions of I-BET151 (10 mM), Ro 11-1464 (10 mM), TSA (6.6 mM), and SAHA (10 mM) were prepared in DMSO (Sigma Aldrich) and stored at −20 °C. Each of the conditioned media was freshly prepared before treatment.
I-BET151, Ro 11-1464, trichostatin A (TSA), and suberoylanilide hydroxamic acid (SAHA) were purchased from Sigma Aldrich (St. Louis, MO, USA). Stock solutions of I-BET151 (10 mM), Ro 11-1464 (10 mM), TSA (6.6 mM), and SAHA (10 mM) were prepared in DMSO (Sigma Aldrich) and stored at −20 °C. Each of the conditioned media was freshly prepared before treatment.