Yeast dna extraction reagent kit

The DNA was extracted from about 10⁸ yeast cells with a Yeast DNA Extraction Reagent Kit according to the manufacturer’s instructions (Thermo Scientific, Illkirch, France). The 8-oxo-guanine content was then examined by the 8-OHdG DNA Damage ELISA Kit according to the manufacturer’s instructions (Cell BioLabs, Paris, France).

DNA was extracted from about 10⁸ yeast cells with Yeast DNA Extraction Reagent Kit following manufacturer’s instructions (Thermo Scientific, Illkirch, France) and 8-oxo-guanine content was determined with the 8-OHdG DNA Damage ELISA kit following the manufacturer’s instructions (Cell BioLabs, Paris, France).

The membrane lipid peroxide was measured using thiobarbituric acid (TBA; Sigma Aldrich, Saint-Quentin Fallavier, France). Approximately 10⁸ yeast cells were crushed in double distilled water and centrifuged at 10,000× g for 10 min. The supernatant (75 µL) was combined with 25 µL of SDS (3% w/v), 50 L of TBA (3% w/v) produced in 50 mM NaOH, and 50 µL of HCl (23% v/v). Between each addition, the mixture was thoroughly mixed. The resulting combination was heated to 80 °C for 20 min, then cooled on ice before measuring the absorbance at 532 nm (A532). Absorbance at 600 nm (i.e., non-specific absorbance measurement) was removed. A standard curve was prepared using 1,1,3,3, tetramethoxypropane to measure the concentrations of TBARS in the samples.
Total proteins were extracted from about 10⁸ yeast cells as described above (Section 4.11). The protein carbonyl content was determined using a Protein Carbonyl ELISA kit following the manufacturer’s instructions (Cell BioLabs, Paris, France).
DNA was extracted from about 10⁸ yeast cells with a Yeast DNA Extraction Reagent Kit following the manufacturer’s instructions (Thermo Scientific, Illkirch, France) and the 8-oxo-guanine content was determined with the 8-OHdG DNA Damage ELISA kit following the manufacturer’s instructions (Cell BioLabs, Paris, France).