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Jm109 de3 cells

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JM109 (DE3) cells are a strain of Escherichia coli (E. coli) bacteria used for the expression of recombinant proteins. These cells are designed for high-level protein expression and are commonly used in molecular biology and biotechnology applications.

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Lab products found in correlation

Pfastbac, by Thermo Fisher Scientific (2 mentions) Rosetta 2 de3 cells, by Merck Group (2 mentions) Hek293t cell line crl 11268, by American Type Culture Collection (2 mentions)

2 protocols using jm109 de3 cells

1

Heterologous Expression and Purification of Ric-8A

Cited 1 time
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Rat GST-TEV-Ric-8A was expressed in Tni (Trichoplusia ni) cells infected with recombinant baculovirus (pFastBac, Invitrogen). Tni cells were obtained from Expression Systems, LLC. Rat GST-TEV-Ric-8A together with human Gαq were expressed in Tni (Trichuplusia ni) cells infected with two recombinant baculoviruses (pFastBac, Invitrogen). Human 6His-3C-Gαi1 WT or 6His-3C-Gαi1 ΔF354 were expressed in Rosetta 2(DE3) cells (Merck Millipore). Human Gαi1 (6His between M119 and T120) was expressed in JM109 (DE3) cells (Promega). MBP-Ric-8A (423–530) was expressed in Rosetta 2 (DE3) cells. The HEK293T cell line (CRL-11268) was obtained from the American Type Culture Collection (ATCC). Crispr/Cas9 procedures were used previously to delete RIC-8A in the HEK293T cells (Papasergi-Scott et al., 2018 (link)). 3X-FLAG-Ric-8A point mutant plasmids were transfected and selected for stable expression with Hygromycin B.
Seven A.B., Hilger D., Papasergi-Scott M.M., Zhang L., Qu Q., Kobilka B.K., Tall G.G, & Skiniotis G. (2020). Structures of Gα Proteins in Complex with Their Chaperone Reveal Quality Control Mechanisms. Cell reports, 30(11), 3699-3709.e6.
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2

Heterologous Expression and Purification of Ric-8A

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Rat GST-TEV-Ric-8A was expressed in Tni (Trichoplusia ni) cells infected with recombinant baculovirus (pFastBac, Invitrogen). Tni cells were obtained from Expression Systems, LLC. Rat GST-TEV-Ric-8A together with human Gαq were expressed in Tni (Trichuplusia ni) cells infected with two recombinant baculoviruses (pFastBac, Invitrogen). Human 6His-3C-Gαi1 WT or 6His-3C-Gαi1 ΔF354 were expressed in Rosetta 2(DE3) cells (Merck Millipore). Human Gαi1 (6His between M119 and T120) was expressed in JM109 (DE3) cells (Promega). MBP-Ric-8A (423–530) was expressed in Rosetta 2 (DE3) cells. The HEK293T cell line (CRL-11268) was obtained from the American Type Culture Collection (ATCC). Crispr/Cas9 procedures were used previously to delete RIC-8A in the HEK293T cells (Papasergi-Scott et al., 2018 (link)). 3X-FLAG-Ric-8A point mutant plasmids were transfected and selected for stable expression with Hygromycin B.
Seven A.B., Hilger D., Papasergi-Scott M.M., Zhang L., Qu Q., Kobilka B.K., Tall G.G, & Skiniotis G. (2020). Structures of Gα Proteins in Complex with Their Chaperone Reveal Quality Control Mechanisms. Cell reports, 30(11), 3699-3709.e6.
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