Cardamonin

Reagents of analytical grade were used as received from their commercial sources, while cardamonin (98% purity) was obtained from Shanghai Yuanye Bio-Technology Co. Ltd (Shanghai, China). Elemental analysis was performed on a Vario MACRO CUBE CHNS analyzer, while thermogravimetric analysis (TGA) was performed via a Mettler Toledo TGA analyser up to 800 o C with a heating rate of 10 o C/min in an atmosphere of N2. IR spectra were recorded by a Perkin Elmer FTIR spectrophotometer within the range (4000-400 cm -1 ) using KBr discs while melting points have been measured using an Electrothermal IA9100 digital m.p. apparatus measuring within a range of (0 o C-400 o C). HPLC purity analysis was performed using an Agilent 1260 Infinity system equipped with a Zorbax 300SB-C18 column (5 µm, 250 mm x 4.6 mm). Mobile solvent A was water, while B was acetonitrile and the flow rate used was 1.25 ml/min The time program used for the HPLC analysis was 55% B (0-5 min), 55% -85% B (5-25 min) and 99% B (25-30 min).
1 H NMR spectra were obtained via a Bruker FT-NMR 300 MHz, 400 MHz and 600 MHz. TLC was performed using 60F aluminium silica gel aluminium plates with 254 nm fluorescent indicator from Merck. UV-Vis spectra were obtained via an UltroSpec 8000 spectrophotometer.