Serum levels of brain natriuretic peptide (BNP), glutathione (GSH), superoxide dismutase (SOD), and lipid peroxidation malondialdehyde (MDA) were measured using a Mouse BNP ELISA Kit (Catalog#M0204c, Elabscience, Wuhan, China), a GSH ELISA Kit (Catalog#0026C, Elabscience, Wuhan, China), Cu/Zn-SOD and Mn-SOD Assay Kit with WST-8 (Catalog#S0103, Beyotime Biotech, Beijing, China), and a Peroxidation MDA Assay Kit (Catalog#S0131S, Beyotime Biotech, Beijing, China), respectively, according to the manufacturer’s instructions.
Gsh elisa kit
Manufactured by Elabscience
Sourced in China
The GSH ELISA Kit is a quantitative immunoassay designed to measure the level of glutathione (GSH) in biological samples. The kit utilizes the sandwich ELISA technique to provide accurate and reliable results.
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4 protocols using gsh elisa kit
1
Oxidative Stress Biomarkers in Mice
2
Glutathione and Malondialdehyde Quantification
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Glutathione and malondialdehyde levels were determined in water-soluble proteins. Total glutathione levels were estimated with the GSH ELISA kit from Elabscience (catalog#: E-EL-0026, Bethesda, MD) according to the manufacturer’s protocol. The GSH concentration was expressed as nanomoles of GSH per milligrams of tissue using standard concentrations of GSH (0.005–0.325 mM). Malondialdehyde levels were estimated with the MDA ELISA kit from Elabscience according to the manufacturer’s protocol (catalog #: E-EL-0060). The MDA concentration was expressed as nanomoles of MDA per grams of tissue using standard concentrations of MDA (0.434–0.1388 μM). GSH and MDA standard curves were drawn using Curve expert professional software (v.2.3.0, Starkville, MS).
3
Oxidative Stress Markers in C2C12 Cells
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According to the manufacturer’s instructions, the levels of MDA (malondialdehyde), GSH (glutathione), and SOD (superoxide dismutase) in C2C12 cells were measured using a GSH ELISA Kit (Elabscience, Wuhan, China), MDA Content Detection Kit (Beyotime, Nantong, China), and SOD Activity Detection Kit (Beyotime, Nantong, China).
4
Sal B Attenuates Ang II-Induced Oxidative Stress
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For the analysis, after being pretreated with 12.5, 25.0, and 50.0 μM Sal B for 2 h, FBs were stimulated by 100 nM Ang II for 24 h. The cell supernatants were then collected for the analysis. The level of glutathione (GSH) was quantified by using the GSH ELISA kit (# E-EL-0026c, Elabscience, Wuhan, China), in accordance with the manufacturer’s instructions. The reactive oxygen species (ROS) level was examined in cells using 2′,7′-dichlorofluorescin diacetate for 30 min at 37 °C. The cells were observed, and data were analyzed through microplate reader.
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