Ab74871

Manufactured by Abcam

Ab74871 is a laboratory equipment product. It is a device used for scientific experiments and analysis in a laboratory setting. The core function of this product is to perform a specific task related to the research or testing being conducted. No further details about the intended use or features of this product can be provided in an unbiased and factual manner.

Automatically generated - may contain errors

Lab products found in correlation

Abs204, by Merck Group (1 mentions) Ab8984, by Abcam (1 mentions) Protein extraction buffer, by Beyotime (1 mentions) Irdye 800 goat anti rabbit igg, by LI COR (1 mentions) Irdye 680 goat anti mouse igg, by LI COR (1 mentions) Ab9485, by Abcam (1 mentions) Ab32572, by Abcam (1 mentions) Nucleoprotein extraction kit, by Sangon (1 mentions)

2 protocols using ab74871

Western Blotting Experimental Details

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blotting experiments were carried out as previously described^{48 (link)}. Anti-HSP90 (610419; BD Biosciences), anti-MPC1 (ab74871; Abcam), anti-phospho-PDHE1α (Ser-293) (ABS204; Millipore) and anti-PDHE1α (#3205; Cell Signaling Technology) antibodies were used at a dilution of 1/1000 (or 1/250 for the anti-MPC1 antibody) in 5% bovine serum albumin (BSA). Anti-MCT1 and anti-MCT4 antibodies (custom-made, ThermoFisher Scientific) were used at 1/1000 in 5% skimmed milk. Uncropped versions of Western blots are presented in Supplementary Fig. 7.

Corbet C., Bastien E., Draoui N., Doix B., Mignion L., Jordan B.F., Marchand A., Vanherck J.C., Chaltin P., Schakman O., Becker H.M., Riant O, & Feron O. (2018). Interruption of lactate uptake by inhibiting mitochondrial pyruvate transport unravels direct antitumor and radiosensitizing effects. Nature Communications, 9, 1208.

+ Open protocol

+ Expand

Subcellular Protein Fractionation and Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole-cell lysates or nuclear protein was extracted using a protein extraction buffer (Beyotime, Shanghai, China) or nucleoprotein extraction kit (Sangon Biotech, C500009), respectively. Proteins were resolved by SDS-PAGE and transferred onto nitrocellulose (NC) membranes using standard methods. Primary antibodies used as follows: MPC1 (ab74871, Abcam), β-catenin (ab32572, Abcam), lamin A/C (ab8984, Abcam), and GAPDH (ab9485, Abcam). Species-specific secondary antibodies used as follows: IRDye 680 Goat anti-Mouse IgG (LI-COR) and IRDye 800 Goat anti-rabbit IgG (LI-COR).

Tian G.A., Xu C.J., Zhou K.X., Zhang Z.G., Gu J.R., Zhang X.L, & Wang Y.H. (2020). MPC1 Deficiency Promotes CRC Liver Metastasis via Facilitating Nuclear Translocation of β-Catenin. Journal of Immunology Research, 2020, 8340329.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!