A549/VIM-RFP cells were obtained from (ATCC CCL-185EMT, RRID:CVCL_LI35 ), and cells within 5–15 generations were used in the studies. Cells were cultured in F-12K medium (Corning) with 10% fetal bovine serum (FBS) in MatTek glass bottom culture dishes (P35G-0–10 C) in a humidified atmosphere at 37 °C and 5% CO2, as detailed in Wang et al., 2020 (link). The cell line was authenticated as A549 cells with short tandem repeat (STR) analysis by University of Arizona Genetics Core (UAGC). The result of mycoplasma contamination test (MycoFluor Mycoplasma Detection Kit Molecular Probes, M-7006) was negative.
Ccl 185emt
Manufactured by American Type Culture Collection
Sourced in United States
The CCL-185EMT is a laboratory equipment product manufactured by American Type Culture Collection. It is designed for cell culture applications. The core function of this product is to facilitate the incubation and growth of cell lines.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ccl 185emt
1
Culturing A549 cells with VIM-RFP
2
ROCK1 Inhibition in NSCLC Cell Lines
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The normal pulmonary epithelial cell line BEAS-2B (American Type Culture Collection (ATCC)® no. CRL-9609™) and the NSCLC cell line A549 (ATCC® no. CCL-185EMT™) were purchased from ATCC (Manassas, VA, USA). The cells were cultured in Low Glucose-Dulbecco's modified Eagle's medium (L-DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing low glucose, 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% streptomycin and penicillin at 37°C in an atmosphere containing 5% CO2. To inhibit ROCK1 activity, Y-27632 (5 mM; cat. no. S1049; Selleck Chemicals, Houston, TX, USA) was added to the medium for 4 h (21 (link)).
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