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Cd32b apc

Manufactured by Thermo Fisher Scientific
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CD32b APC is a lab equipment product that is used for the detection and measurement of CD32b, a cell surface receptor, in various cell types. The product utilizes the Allophycocyanin (APC) fluorescent dye to label the CD32b antibody, enabling its visualization and quantification through flow cytometry or other analytical techniques.

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Lab products found in correlation

Fortessa x20, by BD (2 mentions) Cd45 buv395, by BD (2 mentions) Ly6g pe cy7, by BioLegend (2 mentions) Cd3 apc cy7, by BioLegend (2 mentions) Cd19 bv605, by BioLegend (2 mentions) Macsquant analyzer, by Miltenyi Biotec (2 mentions) Ethylene diamine tetraacetic acid (edta), by Corning (2 mentions) Cd11b percp cy5, by BioLegend (2 mentions) Ly6c pacific blue, by BioLegend (2 mentions) Cd16 fitc, by BioLegend (2 mentions) Nk1.1 pe cy7, by BioLegend (2 mentions) Ly6b fitc, by Abcam (2 mentions) Mhc class 2 a700, by BioLegend (2 mentions) Cd45 buv95, by BioLegend (2 mentions) Cd16.2 apc, by BioLegend (2 mentions) Cd64 apc, by BioLegend (2 mentions)

2 protocols using cd32b apc

1

Isolation and Staining of Myeloid Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For cell depletion experiments, whole blood was harvested from mice and mixed with 5 mM EDTA (Corning). Red blood cells were lysed with ACK lysis buffer at room temperature before being washed and chilled in PBS + 5% FCS. Monocytes were stained with CD45 BUV395 (BD Biosciences clone 30-F11), CD11b PerCP-Cy5.5 (BioLegend clone M1/70), Ly6B FITC (Abcam clone 7/4), Ly6G PE-Cy7 (BioLegend clone 6D5), Ly6C Pacific Blue (BioLegend clone HK1.4) and MHC class II A700 (BioLegend clone M5/114.15.2). NK cells were stained with CD45 BUV95, CD3 APC-Cy-7 (BioLegend clone 145–2C11), CD19 BV605 (BioLegend clone 6D5), and NK1.1 Pe-Cy7 (BioLegend clone PK136). Viability was determined through exclusion of a fixable viability dye (e506;eBiosciences). Samples were fixed and processed on a BD-Fortessa X20. For FcγR expression experiments BV2, LADMAC, and Vero cells were stained with one of the following: CD64 APC (BioLegend clone X54–5/7.1), CD32b APC (Invitrogen clone AT130–2), CD16 FITC (BioLegend clone 221–3A4), CD16.2 APC (BioLegend clone 9E9). Cells were analyzed on a MACSQuant analyzer (Miltenyi). All FACS data were analyzed by FlowJo v. 10.7.
Earnest J.T., Holmes A.C., Basore K., Mack M., Fremont D.H, & Diamond M.S. (2021). The mechanistic basis of protection by non-neutralizing anti-alphavirus antibodies. Cell reports, 35(1), 108962.
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2

Isolation and Staining of Myeloid Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For cell depletion experiments, whole blood was harvested from mice and mixed with 5 mM EDTA (Corning). Red blood cells were lysed with ACK lysis buffer at room temperature before being washed and chilled in PBS + 5% FCS. Monocytes were stained with CD45 BUV395 (BD Biosciences clone 30-F11), CD11b PerCP-Cy5.5 (BioLegend clone M1/70), Ly6B FITC (Abcam clone 7/4), Ly6G PE-Cy7 (BioLegend clone 6D5), Ly6C Pacific Blue (BioLegend clone HK1.4) and MHC class II A700 (BioLegend clone M5/114.15.2). NK cells were stained with CD45 BUV95, CD3 APC-Cy-7 (BioLegend clone 145–2C11), CD19 BV605 (BioLegend clone 6D5), and NK1.1 Pe-Cy7 (BioLegend clone PK136). Viability was determined through exclusion of a fixable viability dye (e506;eBiosciences). Samples were fixed and processed on a BD-Fortessa X20. For FcγR expression experiments BV2, LADMAC, and Vero cells were stained with one of the following: CD64 APC (BioLegend clone X54–5/7.1), CD32b APC (Invitrogen clone AT130–2), CD16 FITC (BioLegend clone 221–3A4), CD16.2 APC (BioLegend clone 9E9). Cells were analyzed on a MACSQuant analyzer (Miltenyi). All FACS data were analyzed by FlowJo v. 10.7.
Earnest J.T., Holmes A.C., Basore K., Mack M., Fremont D.H, & Diamond M.S. (2021). The mechanistic basis of protection by non-neutralizing anti-alphavirus antibodies. Cell reports, 35(1), 108962.
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