Silodosin

Krebs solution was obtained from Sigma Aldrich (St. Louis, MO, USA). Silodosin (Recordati, Milan, Italy), tamsulosin (Cayman, Ann Arbor, MI, USA), and naftopidil (Cayman) were procured and used. Silodosin and tamsulosin were dissolved in ethanol and naftopidil in methanol (Merck, Kenilworth, NJ, USA) and stored as stock solutions: Silodosin (1 mM), tamsulosin (400 nM), and naftopidil (200 µM) [15 (link)16 17 ].

The following chemicals were used: NaCl, NaHCO₃, KCl, CaCl₂, MgCl₂, glucose, NaH₂PO₄, ethylenediaminetetraacetate, nicotine, acetylcholine, lidocaine, silodosin, NE, capsaicin, and PE (all from Sigma-Aldrich, ST Louis, MO, USA). A paired samples t-test was used to compare the difference in the same strip. An analysis of variance followed by post hoc tests using Bonferroni was used to compare the difference between different strips. All values are presented as mean ± standard error of the mean. P < 0.05 was considered statistically significant.

The following chemicals were used: NaCl, NaHCO₃, KCl, CaCl₂, MgCl₂, glucose, NaH₂PO₄, EDTA, acetylcholine, capsaicin, guanethidine, lidocaine, propranolol, silodosin, phenylephrine, and norepinephrine (all from Sigma-Aldrich, St. Louis, MO, USA). All values are presented as mean±standard error of the mean. The paired t-test was used to compare differences in the same strip. P-values of <0.05 were considered to indicate statistical significance. Analysis of variance followed by post hoc testing (Bonferroni) was used to compare differences between strips. The Bonferroni test sets the cutoff for significance at α/n (α=0.05, n=number of groups). Thus, P-values of <0.025 (0.05 of 2) and <0.0167 (0.05 of 3) are statistically significant findings in analyses with 2 and 3 pairs of subjects, respectively.