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Fatty acid free bovine serum albumin

Manufactured by Roche
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Fatty acid-free bovine serum albumin is a purified protein derived from bovine serum. It is used as a stabilizing and blocking agent in various laboratory applications.

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Lab products found in correlation

Collagenase type 1, by Merck Group (4 mentions) Antigen capture elisa kit, by Phoenix Pharmaceuticals (2 mentions) Spexin, by Phoenix Pharmaceuticals (2 mentions) Isobutylmethylxanthine, by Merck Group (1 mentions) 8 bromo cyclic adenosine monophosphate camp, by Merck Group (1 mentions) 3t3 l1 cells, by ZenBio (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Insulin, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Luciferase assay reagent, by Promega (1 mentions) Insulin, by Thermo Fisher Scientific (1 mentions) Epidermal growth factor (egf), by Merck Group (1 mentions) Igf 1, by Thermo Fisher Scientific (1 mentions) Endo free purification kit, by Qiagen (1 mentions) Ag1478, by Merck Group (1 mentions) D 5 3h n glucose, by PerkinElmer (1 mentions) Anti hk2 antibody, by Cell Signaling Technology (1 mentions) Taqman universal pcr master mix, by Thermo Fisher Scientific (1 mentions) 2 deoxy d glucose 2 dg, by Merck Group (1 mentions) Transit tko, by Mirus Bio (1 mentions)

Close spelling variants of this product

Bovine Serum Albumin Fraction V. fatty acid free Bovine serum

6 protocols using fatty acid free bovine serum albumin

1

Spexin and Leptin Quantification

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[3H]-oleic acid (OA) was from NEN Life Science Products (Boston, MA, USA), type I collagenase from Sigma (St Louis, MO, USA), and fatty acid-free bovine serum albumin (BSA) from Boehringer Mannheim (Indianapolis, IN). Circulating Spexin was assayed with a competitive EIA kit and leptin by an antigen capture ELISA kit from Phoenix Pharmaceuticals (Burlingame, CA).
Ge F., Walewski J.L., Torghabeh M.H., Lobdell H I.V., Hu C., Zhou S., Dakin G., Pomp A., Bessler M., Schrope B., Ude-Welcome A., Inabnet W.B., Feng T., Carras-Terzian E., Anglade D., Ebel F.E, & Berk P.D. (2015). Facilitated Long Chain Fatty Acid Uptake by Adipocytes Remains Upregulated Relative to BMI for More Than a Year After Major Bariatric Surgical Weight Loss. Obesity (Silver Spring, Md.), 24(1), 113-122.
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2

Spexin and Leptin Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
[3H]-oleic acid (OA) was from NEN Life Science Products (Boston, MA, USA), type I collagenase from Sigma (St Louis, MO, USA), and fatty acid-free bovine serum albumin (BSA) from Boehringer Mannheim (Indianapolis, IN). Circulating Spexin was assayed with a competitive EIA kit and leptin by an antigen capture ELISA kit from Phoenix Pharmaceuticals (Burlingame, CA).
Ge F., Walewski J.L., Torghabeh M.H., Lobdell H I.V., Hu C., Zhou S., Dakin G., Pomp A., Bessler M., Schrope B., Ude-Welcome A., Inabnet W.B., Feng T., Carras-Terzian E., Anglade D., Ebel F.E, & Berk P.D. (2015). Facilitated Long Chain Fatty Acid Uptake by Adipocytes Remains Upregulated Relative to BMI for More Than a Year After Major Bariatric Surgical Weight Loss. Obesity (Silver Spring, Md.), 24(1), 113-122.
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3

Radiolabeled Oleic Acid and Spexin Protocols

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9,10-[3H]-Oleic acid (OA) was purchased from NEN Life Science Products (Boston, MA, USA), type I collagenase from Sigma (St. Louis, MO, USA), and fatty acid free bovine serum albumin (BSA) from Boehringer Mannheim (Indianapolis, IN, USA).
Two preparations of Spexin were used: the first from Phoenix Pharmaceuticals (Burlingame, CA) and the second, a custom synthesis product from the Ferring Research Institute (San Diego, CA). Both were >99% pure by HPLC and ID'd by LC/MS.
Walewski J.L., Ge F., Lobdell H I.V., Levin N., Schwartz G.J., Vasselli J., Pomp A., Dakin G, & Berk P.D. (2014). Spexin is a Novel Human Peptide that Reduces Adipocyte Uptake of Long Chain Fatty Acids and Causes Weight Loss in Rodents with Diet-induced Obesity. Obesity (Silver Spring, Md.), 22(7), 1643-1652.
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4

Radiolabeled Oleic Acid and Spexin Protocols

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9,10-[3H]-Oleic acid (OA) was purchased from NEN Life Science Products (Boston, MA, USA), type I collagenase from Sigma (St. Louis, MO, USA), and fatty acid free bovine serum albumin (BSA) from Boehringer Mannheim (Indianapolis, IN, USA).
Two preparations of Spexin were used: the first from Phoenix Pharmaceuticals (Burlingame, CA) and the second, a custom synthesis product from the Ferring Research Institute (San Diego, CA). Both were >99% pure by HPLC and ID'd by LC/MS.
Walewski J.L., Ge F., Lobdell H I.V., Levin N., Schwartz G.J., Vasselli J., Pomp A., Dakin G, & Berk P.D. (2014). Spexin is a Novel Human Peptide that Reduces Adipocyte Uptake of Long Chain Fatty Acids and Causes Weight Loss in Rodents with Diet-induced Obesity. Obesity (Silver Spring, Md.), 22(7), 1643-1652.
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5

3T3-L1 Adipocyte Differentiation Protocol

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3T3-L1 cells were purchased from Zen-Bio (Research Triangle Park, NC, USA). Dulbecco's modified Eagle's medium and other culture reagents were purchased from Thermo Scientific (Rockford, IL, USA). Fatty acid-free bovine serum albumin was purchased from Roche Molecular Biochemicals (Indianapolis, IN, USA). Isobutyl methyl xanthine, dexamethasone, insulin, isoproterenol (ISOP) and 8-Bromo-cyclic adenosine monophosphate (cAMP) were purchased from Sigma (St Louis, MO, USA). Human HDL was obtained as a gift from Dr Jay Heinecke (Department of Medicine, University of Washington, Seattle, WA, USA), and the method of HDL preparation has been published previously.26 (link) Human apoA-I was purified as previously described.27 (link)
Wei H., Averill M.M., McMillen T.S., Dastvan F., Mitra P., Subramanian S., Tang C., Chait A, & LeBoeuf R.C. (2014). Modulation of adipose tissue lipolysis and body weight by high-density lipoproteins in mice. Nutrition & Diabetes, 4(2), e108-.
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6

Glucose Uptake Regulation by LPA

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LPA (1-oleoly, 18:1) was obtained from Avanti Polar Lipids, Inc. (Alabaster, AL). Prior to use, LPA was dissolved in PBS containing 0.5% fatty acid-free bovine serum albumin (BSA) from Roche (Indianapolis, IN). d-[5-3H(N)]-glucose was purchased from Perkin Elmer (Boston, MA). EGF, 2-deoxy-d-glucose (2-DG), and AG 1478 were obtained from Sigma Aldrich (St. Louis, MO). IGF-1 and insulin were from Invitrogen (Gaithersburg, MD). Plasmid DNAs were purified using the endo-free purification kit from Qiagen (Valencia, CA). Dharmafect 1 was obtained from Dharmacon, Inc. (Lafayette, CO) and TransIT-TKO was obtained from Mirus Bio (Madison, WI). Luciferase assay reagents were obtained from Promega (Madison, WI). Anti-HK2 antibody was obtained from Cell Signaling (Danvers, MA). The TaqMan Universal PCR Master Mix and qPCR probes for HK2 and GAPDH were obtained from Applied Biosystems (Carlsbad, CA).
Mukherjee A., Ma Y., Yuan F., Gong Y., Fang Z., Mohamed E.M., Berrios E., Shao H, & Fang X. (2015). Lysophosphatidic Acid Up-Regulates Hexokinase II and Glycolysis to Promote Proliferation of Ovarian Cancer Cells. Neoplasia (New York, N.Y.), 17(9), 723-734.
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