Anti human cd14s percp cy5.5 conjugated antibodies

Blood samples needed for flow cytometry and biochemical analysis were collected from all participants in the morning period, after at least a 10 h fast. All samples were analyzed in a single laboratory by an experienced biochemist following the same standard procedure. The biochemist was blinded for the participant’s assignment to Crohn’s disease or ulcerative colitis group. Blood was drawn from a polyethylene catheter inserted into the antecubital vein. One hundred microliters of the whole blood was pre-treated with an Fc receptor-blocking reagent (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) to prevent nonspecific binding and was incubated for 20 min in the dark at 25 °C with 4 µL of anti-human-CD14s PerCP-Cy5.5-conjugated antibodies (eBioscience, San Diego, CA, USA), 4 µL of phycoerythrin-conjugated antibodies reactive to human CD16 (eBioscience, San Diego, CA, USA), and 10 µL of mouse antibodies reactive to human CD44 conjugated with FITC (BD Pharmingen, San Diego, CA, USA). Following the red blood cell lysis with lysis solution (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), cells were analyzed by flow cytometry (BD Accuri C6, BD Biosciences, Aalst, Belgium). Unstained cell samples were measured and processed as negative controls to set the appropriate regions. Cell acquisition was stopped at 10⁶ cells.