Ab83250

Manufactured by Abcam

Ab83250 is a high-quality primary antibody used in various research applications. It is a purified rabbit monoclonal antibody that specifically recognizes the target antigen. The product is supplied in a buffered solution and is suitable for techniques such as Western blotting, immunohistochemistry, and ELISA. Further details about the intended use or performance characteristics of this product are not available.

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Lab products found in correlation

Ab21667, by Abcam (1 mentions) Ab120812, by Abcam (1 mentions) Ccl22, by R&D Systems (1 mentions) Recombinant human ccl2, by BioLegend (1 mentions) Anti akt antibody 9272, by Cell Signaling Technology (1 mentions) Azd5363 s8019, by Selleck Chemicals (1 mentions) Anti gapdh antibody nb300 221, by Novus Biologicals (1 mentions) Ab56023, by Abcam (1 mentions) Ab22552, by Abcam (1 mentions) Alizarin red solution, by Thermo Fisher Scientific (1 mentions) Dfc 3200 camera, by Leica (1 mentions) Ckx41 microscope, by Leica (1 mentions) Ab150077, by Abcam (1 mentions)

2 protocols using ab83250

Evaluating Chemokine Receptor Signaling Pathways

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Recombinant human CCL17 and CCL22 were purchased from R&D Systems (Minneapolis, MN). Recombinant human CCL2 was obtained from BioLegend (San Diego, CA). Antibodies used in western blotting, IHC, immunocytochemistry, and flow cytometry were as follows: rabbit anti-human CCR2 antibody (ab21667), rabbit anti-human CCR4 antibody (ab83250), goat anti-human CCR4 antibody (ab1669), rabbit anti-goat immunoglobulin (Ig)G H&L (fluorescein isothiocyanate; FITC) antibody (6737), goat anti-rabbit IgG H&L (FITC) antibody (6717), anti-mannose receptor antibody (ab64693), and anti-CCR7 antibody (ab103404) obtained from Abcam (Cambridge, MA); anti-Akt antibody (9272), anti-phospho-Akt (Thr308) antibody (9275), anti-phospho-Akt (Ser473) antibody (9271), and anti-rabbit IgG HRP-linked antibody (7074) obtained from Cell Signaling Technology (Danvers, MA); and anti-GAPDH antibody (NB300-221) obtained from Novus Biologicals (Littleton, CO). The antagonists used in the neutralizing assay were a selective CCR2 antagonist (ab120812) obtained from Abcam and a CCR4 antagonist (SC-221406) obtained from Santa Cruz Biotechnology (Dallas, TX). The Akt inhibitor AZD5363 (S8019) was obtained from Selleckchem (Houston, TX).

Maolake A., Izumi K., Shigehara K., Natsagdorj A., Iwamoto H., Kadomoto S., Takezawa Y., Machioka K., Narimoto K., Namiki M., Lin W.J., Wufuer G, & Mizokami A. (2016). Tumor-associated macrophages promote prostate cancer migration through activation of the CCL22–CCR4 axis. Oncotarget, 8(6), 9739-9751.

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Histochemical Analysis of Calcification

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Hematoxylin and Eosin (H&E) staining was performed according to routine Harris H&E protocol. For alizarin red staining, slides were stained with alizarin red solution (Alfa Aesar, Haverhill, MA, USA) for 45 s. The extent of calcification was evaluated by the ratio (%) of the calcium staining area to the total area, which could be calculated with the Image J version 1.5.0 software (National Institutes of Health). There were totally four region of interest every group and blinding was not performed. For Immunofluorescence (IF) staining, sections were blocked in 10% bovine serum albumin for 30 min and then incubated overnight at 4°C with mixed primary antibodies against ALP (ab83250, Abcam), OSX (ab22552, Abcam) and BMP-7 (ab56023, Abcam). Sections were incubated with fluorescein isothiocyanate-conjugated secondary antibody (ab150077, Abcam) for 1 h, then mounted with 4′, 6-diamidino-2-phenylindole (DAPI) on second day. The images were taken by an Olympus CKX41 microscope with a Leica DFC 3200 camera.

Cao G., L.i., Xiang S., Lin H., Pei F., Tuan R.S.C, & Alexander P.G. (2023). The development of a mouse model to investigate the formation of heterotopic ossification. Journal of orthopaedic surgery (Hong Kong), 31(1).

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