Nucleic acid samples were treated with DNaseI (New England Biolabs) at 37°C, and samples were analyzed on 1.2% TAE agarose gel stained with ethidium bromide. For subsequent analyses, treated samples were column purified using standard PCR purification kits (Qiagen), reverse transcribed with Maxima First-Strand cDNA Synthesis Kit (Thermo Fisher Scientific), and subjected to PCR to test for the presence/absence of various genomic and viral elements, as described above. PCR samples were analyzed on 2% TBE (Tris-borate EDTA) gels at 4–6 V/cm for 75–90 minutes in prechilled 0.5× TBE buffer.
Standard pcr purification kits
Manufactured by Qiagen
The Standard PCR purification kits are laboratory equipment designed to efficiently purify PCR amplicons. The kits utilize a simple and reliable spin-column procedure to remove unwanted components from PCR reactions, allowing for the recovery of high-quality DNA fragments suitable for downstream applications.
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Lab products found in correlation
2 protocols using standard pcr purification kits
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Nucleic Acid Purification and Analysis
2
Nucleic Acid Analysis Workflow
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Nucleic acid samples were treated with DNaseI, RNaseIf, RNaseH, or ShortCut RNaseIII (all from NEB) at 37°C and samples were analyzed on 1.2% TAE-agarose gel stained with ethidium bromide. For subsequent analyses, treated samples were column purified using standard PCR purification kits (Qiagen), reverse transcribed with Maxima first-strand cDNA synthesis kit (Thermo), and subjected to PCR to test for the presence/absence of various genomic and viral elements, as described above. PCR samples were analyzed on 2% TBE (Tris-borate EDTA) gels at 4–6 V/cm for 75–90 min in prechilled 0.5 × TBE buffer.
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