Mir 142 3p

Manufactured by Qiagen

Sourced in United States

MiR-142–3p is a laboratory detection product offered by Qiagen. It is designed to detect the presence and expression levels of the miR-142–3p microRNA target sequence.

Automatically generated - may contain errors

Lab products found in correlation

Mir 30b, by Qiagen (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Miscript 2 rt kit, by Qiagen (1 mentions) β actin, by Merck Group (1 mentions) Mirneasy kit, by Qiagen (1 mentions) Mir 24, by Qiagen (1 mentions) Mirna mimic, by Qiagen (1 mentions) Red siglo oligos, by Thermo Fisher Scientific (1 mentions) Control mirna mimic, by Qiagen (1 mentions)

3 protocols using mir 142 3p

Extracellular Vesicle miRNA and mRNA Profiling

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Total RNA was isolated from EV pellets collected from donor plasma using miRNeasy kit (Qiagen). Seventy-five nanograms of RNA were reverse transcribed using miScript RT II kit (Qiagen) using HiFlex buffer to amplify miRNA and mRNA concurrently. Expression of miR-24, miR-30b-5p, and miR-142–3p (primers purchased from Qiagen), PD-L1 and β-actin (primers purchased from Sigma) was assessed by real-time quantitative PCR, and average Ct values were plotted.

Lema D.A., Jankowska‐Gan E., Nair A., Kanaan S.B., Little C.J., Foley D.P., Raza Naqvi A., Wang J., Hong S., Nelson J.L., Al‐Adra D., Burlingham W.J, & Sullivan J.A. (2022). Cross‐decoration of dendritic cells by non‐inherited maternal antigen‐containing extracellular vesicles: Potential mechanism for PD‐L1‐based tolerance in cord blood and organ transplantation. American Journal of Transplantation, 22(5), 1329-1338.

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miRNA Transfection in Immune Cells

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MiR-24, miR-30b, and miR-142-3p mimics or inhibitors were purchased from Qiagen (Gaithersburg, MD, USA). As a negative control, miRNA mimics with no known human targets was used (Qiagen). Transient transfection of miRNA mimics, inhibitors or control was performed using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). MΦ were transfected at a final concentration of 50 nM oligos while DC were transfected with 100 nM oligos. As a positive transfection control, we used DY-547 labelled siRNA (Life Technologies).

Naqvi A.R., Fordham J.B., Ganesh B, & Nares S. (2016). miR-24, miR-30b and miR-142-3p interfere with antigen processing and presentation by primary macrophages and dendritic cells. Scientific Reports, 6, 32925.

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Overexpressing miRNA in Differentiated Macrophages and Dendritic Cells

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miR-30b, miR-142–3p and control miRNA mimic were purchased from Qiagen (Gaithersburg, MD, USA). Transient miRNA overexpression was performed by transfecting miRNA mimics using Lipofectamine 2000 (Invitrogen-Life Technologies Corporation, Carlsbad, CA, USA) according to the manufacturer’s instructions. Day 7-differentiated MΦ or DC were transfected at a final concentration of 50 nM. Red siGLO oligos (ThermoFisher Scientific, Grand Island, NY, USA) were used to determine transfection efficiency.

Valverde A., Nares S, & Naqvi A.R. (2020). Impaired cell migration and structural defects in myeloid cells overexpressing miR-30b and miR-142–3p. Biochimica et biophysica acta. Gene regulatory mechanisms, 1863(11), 194628.

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