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Base solution

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Base solution is a general-purpose laboratory reagent used to adjust the pH of solutions. It is a clear, colorless liquid that provides a basic environment for various chemical reactions and experiments. The core function of the base solution is to neutralize acidic substances and increase the pH of a solution.

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Lab products found in correlation

Clariostar plus, by BMG Labtech (2 mentions) Nadp nadph glo, by Promega (2 mentions) Nad nadh assay kit, by Promega (2 mentions)

Spelling variants of this product

Tris base solution (6 citations) Fast Garnet GBC base solution (4 citations) Trizma base solution (3 citations) Pre-warmed tissue stain base solution (2 citations)

2 protocols using base solution

1

Quantifying Cellular Redox Ratios

Check if the same lab product or an alternative is used in the 5 most similar protocols
NADP+/NADPH and NAD/NADH ratios were measured using the NADP+/NADPH-GLO and NAD+/NADH assay kit (Promega) respectively following the manufacturer’s instructions. Briefly, 5 x 106 H9C2 cells or 30 mg of heart tissue were lysed in 200 μl or 300 μl respectively of base solution (Sigma). Half of the lysate was treated with 0.4 N HCl at 60 °C in order to remove the NADH and NADPH to allow measurement of NAD+ and NADP+. Cell and tissue lysates were mixed with kit detection reagents and after 30 min, NADP+/NADPH ratios were measured using CLARIOstar® Plus (BMG, Labtech).
Fernandez-Caggiano M., Kamynina A., Francois A.A., Prysyazhna O., Eykyn T.R., Krasemann S., Crespo-Leiro M.G., Vieites M.G., Bianchi K., Morales V., Domenech N, & Eaton P. (2020). Mitochondrial pyruvate carrier abundance mediates pathological cardiac hypertrophy. Nature metabolism, 2(11), 1223-1231.
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2

Quantifying Cellular Redox Ratios

Check if the same lab product or an alternative is used in the 5 most similar protocols
NADP+/NADPH and NAD/NADH ratios were measured using the NADP+/NADPH-GLO and NAD+/NADH assay kit (Promega) respectively following the manufacturer’s instructions. Briefly, 5 x 106 H9C2 cells or 30 mg of heart tissue were lysed in 200 μl or 300 μl respectively of base solution (Sigma). Half of the lysate was treated with 0.4 N HCl at 60 °C in order to remove the NADH and NADPH to allow measurement of NAD+ and NADP+. Cell and tissue lysates were mixed with kit detection reagents and after 30 min, NADP+/NADPH ratios were measured using CLARIOstar® Plus (BMG, Labtech).
Fernandez-Caggiano M., Kamynina A., Francois A.A., Prysyazhna O., Eykyn T.R., Krasemann S., Crespo-Leiro M.G., Vieites M.G., Bianchi K., Morales V., Domenech N, & Eaton P. (2020). Mitochondrial pyruvate carrier abundance mediates pathological cardiac hypertrophy. Nature metabolism, 2(11), 1223-1231.
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