Folin ciocalteu s reagent

2-Diphenyl-1-picrylhydrazyl (DPPH), sodium acetate and quercetin were provided from Sigma Aldrich Chemical Co. (St Louis, MO, USA). Aluminium chloride, citric acid (≥99.7 %), ethylene glycol (≥99.5 %), fructose, glycerol, oxalic acid, urea (≥99 %), and sodium carbonate were taken from Kemaus (New South Wales, Australia). Folin-Ciocalteu's reagent and choline chloride (≥98 %) were obtained from Loba Chemie Pvt. Ltd. (Mumbai, India). Methanol (AR grade) and ethanol (AR grade) were purchased from RCI Labscan Ltd. (Bangkok, Thailand). Gallic acid (>99 %) was acquired from Merck Co. (Weiterstadt, Germany).

The main chemicals and reagents, namely Folin–Ciocalteu’s reagent, were purchased from Loba Chemie PVT.LTD., Mumbai, India. 2,2-diphenyl-1-picryl hydrazyl (DPPH), 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS), 2,4,6-tripyridyl-s-triazine (TPTZ), fluorescein solution, and Lipopolysaccharide (LPS) solution were purchased from Sigma- Aldrich, Darmstadt, Germany. The gallic acid, Trolox, L-Ascorbic acid, Quercetin, and Rutin were HPLC grade and purchased from Sigma-Aldrich, Darmstadt, Germany. 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH) was purchased from FUJIFILM Wako Pure Chemical Corporation, Miyazaki, Japan. Methanol, acetonitrile, and acetic acid were HPLC grade and purchased from RCI Labscan, Bangkok, Thailand, and fetal bovine serum (FBS), penicillin–streptomycin, trypsin-EDTA solution, and RPMI 1640 medium were purchased from Gibco, Waltham, MA USA. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Invitrogen, Waltham, MA, USA.