Eosin phloxine 515

Manufactured by Leica

Eosin Phloxine 515 is a laboratory staining solution used in histology and cytology. It is composed of a combination of eosin and phloxine dyes, which are commonly used for the staining of biological samples. The solution is designed to provide contrast and enhance the visualization of cellular and tissue structures during microscopic examination.

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Lab products found in correlation

Hematoxylin 560 mx, by Leica (2 mentions) Bond polymer refine dab detection kit, by Leica (1 mentions) Bond 3, by Leica (1 mentions) Define mx aq, by Leica (1 mentions)

2 protocols using eosin phloxine 515

Multiparameter Oxidative Stress Analysis

Cited 1 time

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Staining with MitoSox Red and H₂DCF-DA followed by flow cytometry was as previously described (Song et al., 2015 (link)). Phase contrast and fluorescence images were taken with EVOS FL Auto (Bio-Rad). Immunohistochemical staining of tumors tissues were performed with anti-8-ox-dG (Trevigen, Cat # 4354-MC-050), cleaved caapse-3 (Cell Signaling, Cat # 9661) and Ki67 (Leica, Cat # NCL-L-Ki67-MM1) antibodies. Hematoxylin 560 MX (Leica Biosystems, Cat # 3801575) and Eosin Phloxine 515 (Leica Biosystems, Cat# 3801606) were used for H&E staining.

Song J.H., Kang H.J., Luevano L.A., Gokhale V., Wu K., Pandey R., Chow H.H., Hurley L.H, & Kraft A.S. (2019). Small-molecule targeting hairpin loop of hTERT promoter G-quadruplex induces cancer cell death. Cell chemical biology, 26(8), 1110-1121.e4.

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Immunohistochemical Analysis of HNSCC

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The hematoxylin and eosin (H&E) and CD8 IHC staining on HNSCC specimens was performed at UPMC Developmental Laboratory. Slides were cut at 4μm then baked for one hour at 60 degrees Celsius. The slides were cooled to room temperature then deparaffinized and hydrated in diH20. Slides were stained using Hematoxylin 560 MX (3801576, Eosin Phloxine 515 (3801606), Define MX-aq (3803598) and Blue Buffer 8 (3802918) (all catalog numbers from Leica Biosystems). The predilute CD8 (Leica Biosystems, PA0183) was stained on the Bond III instrument (Leica Biosystems) for 8 min. Prior to antibody placement, the antigen retrieval used was the ER2 (pH 8.9–9.1, Leica Biosystems, AR9640) for 20 minutes at 95 deg. Followed by the Bond Polymer Refine Detection Kit-DAB (Leica Biosystems, DS9800).

Luke J.J., Dadey R.E., Augustin R.C., Newman S., Singh K.B., Doerfler R., Behr S., Lee P., Isett B., Deitrick C., Li A., Joy M., Reeder C., Smith K., Urban J., Sellitto L., Jelinek M., Christner S.M., Beumer J.H., Villaruz L.C., Kulkarni A., Davar D., Poklepovic A.S., Najjar Y., Zandberg D.P., Soloff A.C., Bruno T.C., Vujanović L., Skinner H.D., Ferris R.L, & Bao R. (2023). Tumor cell p38 inhibition to overcome immunotherapy resistance. Research Square.

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