Csb e14362m

Manufactured by Cusabio

Sourced in United States

The CSB-E14362m is a laboratory equipment product. It is designed to perform a specific function in a laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

Sta 320, by Cell Biolabs (1 mentions) Csb e13235h, by Cusabio (1 mentions)

3 protocols using csb e14362m

Serum Klotho and 8-OHdG Quantification

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Enzyme-linked immunosorbent assay (ELISA) kits were used to detect Klotho (CSB-E14362m; Cusabio Technology, Houston, TX, USA) and 8-OHdG (STA-320; Cell Biolabs, San Diego, CA, USA) in serum, according to the manufacturer’s instruction.

Lim S.W., Shin Y.J., Luo K., Quan Y., Cui S., Ko E.J., Chung B.H, & Yang C.W. (2019). Ginseng increases Klotho expression by FoxO3-mediated manganese superoxide dismutase in a mouse model of tacrolimus-induced renal injury. Aging (Albany NY), 11(15), 5548-5569.

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Age-Dependent Serum Klotho Levels

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A total of 59 human serum samples were tested in the ELISA assay. They were categorized into two groups according to the age of individuals: Young (N = 23, aged 20–30), and Old (N = 36, aged ≥60). The serum concentrations of Klotho (ELISA mskbio [KE10007]) was examined using ELISA kits according to the manufacturer's instructions. After 3 h of incubation at 37°C, the optical density of each well was determined using a microplate reader at 450 nm. For each assay, the serum sample was diluted in a range of 1:1–1:100 into sample diluents. Duplicate assays were performed for each sample. The levels of Klotho (Cusabio, CSB‐E14362m), GDF15 (Cusabio, CSB‐EL009345MO) and CXCL9 in mice serum were quantified using ELISA kits (Cusabio, CSB‐EL006252MO) according to the manufacturer's protocol.

Zhao X., Zhou S., Liu Y., Gong C., Xiang L., Li S., Wang P., Wang Y., Sun L., Zhang Q, & Yang Y. (2023). Parishin alleviates vascular ageing in mice by upregulation of Klotho. Journal of Cellular and Molecular Medicine, 27(10), 1398-1409.

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Measurement of Serum Klotho and Sirt6

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Serum levels of Klotho or Sirt6 were determined using the following kits for mouse samples (CSB-E14362m, Cusabio technology and OKCD02892, Aviva Systems Biology) and human samples (CSB-E17018h and CSB-E13235h; both Cusabio technology) following the provided protocols. Mouse fetal blood was collected from the trunk of decapitated E18.5 fetuses. Mouse blood from adult animals was sampled from the vena saphena. Human serum samples were obtained from a previous study (10 (link)).

Rudloff S., Bileck A., Janker L., Wanner N., Liaukouskaya N., Lundby C., Huber T.B., Gerner C, & Huynh-Do U. (2021). Dichotomous Responses to Chronic Fetal Hypoxia Lead to a Predetermined Aging Phenotype. Molecular & Cellular Proteomics : MCP, 21(2), 100190.

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