Hpa0354863 100ul

Manufactured by Abcam

HPA0354863-100UL is a laboratory reagent product. It is a 100 microliter vial containing an antibody targeting a specific protein. The core function of this product is to provide researchers with a tool to detect and study the targeted protein in their experiments.

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Lab products found in correlation

Ecl western blotting detection reagent, by Thermo Fisher Scientific (2 mentions) Bradford assay, by Merck Group (2 mentions) Ab6721, by Abcam (2 mentions) Ab97023, by Abcam (2 mentions) Ab5408, by Abcam (2 mentions) Gbox system, by Thermo Fisher Scientific (1 mentions)

2 protocols using hpa0354863 100ul

Protein Quantification and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols

The total protein concentration was determined by Bradford Assay (Sigma) following the manufacturer’s instructions. Cell lysates were heat-denatured and resolved by SDS-PAGE. The membrane was probed against the endogenous proteins by incubation with primary Rabbit anti-myosin VI (1:500, Atlas-Sigma HPA0354863-100UL) or Mouse anti-RNAPII phospho Ser5 (1:500, Abcam Ab5408) and subsequently secondary Goat anti-rabbit antibody (1:15000 Abcam ab6721) or Goat anti-mouse antibody (1:15000, Abcam ab97023) coupled to horseradish peroxidase. The bands were visualised using the ECL Western Blotting Detection Reagents (Invitrogen) and the images were taken using Syngene GBox system. Images were processed in ImageJ.

Hari-Gupta Y., Fili N., dos Santos Á., Cook A.W., Gough R.E., Reed H.C., Wang L., Aaron J., Venit T., Wait E., Grosse-Berkenbusch A., Gebhardt J.C., Percipalle P., Chew T.L., Martin-Fernandez M, & Toseland C.P. (2022). Myosin VI regulates the spatial organisation of mammalian transcription initiation. Nature Communications, 13, 1346.

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Western Blot Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The total protein concentration was determined by Bradford Assay (Sigma) following the manufacturer's instructions. Cell lysates were heat-denatured and resolved by SDS-PAGE. The membrane was probed against the endogenous proteins by incubation with primary Rabbit anti-myosin VI (1:500, Atlas-Sigma HPA0354863-100UL) or Mouse anti-RNAPII phospho Ser5 (1:500, Abcam Ab5408) and subsequently secondary Goat anti-rabbit antibody (1:15000 Abcam ab6721) or Goat anti-mouse antibody (1:15000, Abcam ab97023) coupled to horseradish peroxidase. The bands were visualised using the ECL Western Blotting Detection Reagents (Invitrogen) and the images were taken using Syngene GBox system. Images were processed in ImageJ.

Hari‐Gupta Y., Fili N., Santos Á.d., Cook A.W., Gough R.E., Reed H., Wang L., Aaron J., Venit T., Wait E., Große-Berkenbusch A., Rief M., Percipalle P., Chew T., Martin-Fernandez M.L., & Toseland C.P. (2020). Nuclear myosin VI regulates the spatial organization of mammalian transcription initiation.

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