Animal protocols were approved by the Earle A. Chiles Research Institute (EACRI) Institutional Animal Care and Use Committee (Animal Welfare Assurance No. A3913-01). All experiments were performed in accordance with relevant guidelines and regulations and is reported in accordance with ARRIVE guidelines. Experiments utilized 6–8 week old BALB/c and C57BL/6 mice that were obtained from The Jackson Laboratories. Survival experiments were performed with 6–10 mice per experimental group, and mechanistic experiments with 4–6 mice per group. The CT26 murine colorectal carcinoma cell line26 (link) was obtained from ATCC (Manassas, VA). The Panc02 murine pancreatic adenocarcinoma27 (link) cell line was kindly provided by Dr. Savio Woo (Mount Sinai School of Medicine, New York, NY). The Moc1 murine oral squamous cell carcinoma line28 (link) was kindly provided by Dr. Ravindra Uppaluri at the Dana Faber Cancer Institute. Cell lines were grown in complete RPMI containing 10% heat inactivated fetal bovine serum (FBS), 100U/mL penicillin, 100 µg/mL streptomycin.
Ct26 murine colorectal carcinoma cell line
Manufactured by American Type Culture Collection
The CT26 murine colorectal carcinoma cell line is a well-characterized model for colorectal cancer research. It is a clonal, spontaneously arising adenocarcinoma cell line derived from a BALB/c mouse. The CT26 cell line exhibits consistent growth patterns and is commonly used to study tumor biology, immune response, and therapeutic interventions.
Automatically generated - may contain errors
2 protocols using ct26 murine colorectal carcinoma cell line
1
Murine Cancer Models for Immunotherapy
2
Synthesis and Characterization of Fluorinated Surfactants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Perfluorooctyl bromide (PFOB) were purchased from ABCR GmbH (Germany). Tributyl O-acetylcitrate or acetyl tributyl citrate (ATBC), acetonitrile, and methanol were purchased from Sigma-Aldrich Chemical (Saint-Quentin Fallavier, France). Paclitaxel was obtained from Chemieliva Pharmaceutical Co., Ltd. Dulbecoo's minimal essential serum, fetal bovine serum and penicillin streptomycin, trypsin (1X) and trypan blue were purchased from ThermoFisher Scientific (Gibco, France). The CT26-murine colorectal carcinoma cell line was purchased from ATCC. Fluorinated surfactants, F n d i TAC m , possess a fluorinated chain length of n = 6 carbons with a double polar chain (i = 2) made of a repetition of m = 12 tris(hydroxymethyl)aminomethane (TAC) water soluble units. F 6 d 2 TAC 12 is a member of a recently developed new class of biocompatible branched surfactants called "Dendri-TAC" 26 . Their convergent synthesis allows stepwise modifications of the surfactant's size, shape, hydrophilic/lipophilic balance, nature of the head, number of tails, spacers or connecting units between hydrophilic head and hydrophobic tail. Their highly versatile structure affords not only to control the formation of structurally well-defined macromolecules but also to adjust step-bystep their self-assembling properties.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!