Z lehd fmk

Z-LEHD-FMK (Caspase 9 inhibitor) was purchased from MedChemExpress (MCE), and the Cat. No. is HY-P1010.AF cells were seeded in 12-well culture plates at a density of 5 × 10⁵ cell/plate. All the plates were divided into four groups, with three plates each group. When the confluences of cells reached 80-90%, cell culture media were changed. The cells in one group were pretreated with 6 μl Z-LEHD-FMK for 2 h after being infected with Smad7 overexpression adenovirus. The other three groups were infected with Smad7 overexpression adenovirus, empty adenovirus, or nothing. Cells were collected after 48 h to test apoptosis rate.

The human gastric epithelial cell line GES‐1, and human gastric cancer cell lines, such as SGC7901, MGC803 and HGC27 cells, were obtained from the Cell Culture Centre, Beijing Institute of Basic Medical Science of the Chinese Academy of Medical Science (Beijing, China). The cells were cultured in RPMI Medium 1640 (Gibco, Grand Island, NY, USA) containing 10% foetal bovine serum (FBS, Kangyuan Biology, China). JS‐K was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and dissolved in Dimethyl Sulfoxide (DMSO). Z‐VAD‐FMK (50 μmol/L), Z‐LEHD‐FMK (50 μmol/L) and Z‐DEVD‐FMK (50 μmol/L) were purchased from Medchem Express (Beijing, China). 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide (MTT), carboxy‐PTIO (100 μmol/L) and NAC (500 μmol/L) were purchased from Sigma‐Aldrich (St. Louis, MO, USA).