Bone marrow or peripheral blood samples were obtained from MM patients or healthy donors with written informed consent after approval of the Institutional Review Board of the Dana-Farber Cancer Institute or Kumamoto University in accordance with Declaration of Helsinki. Mononuclear cells were isolated from samples by Ficoll-Paque PLUS (GE Healthcare). MM cells were enriched by anti-CD138 magnetic activated cell separation microbeads (Miltenyi Biotec). Normal B cells from healthy volunteers’ peripheral blood were enriched by negative selection methods using EasySep Human B Cell Isolation Kit (STEMCELL Technologies). For B cell proliferation, isolated B cells were stimulated by 10 μg/ml of human CD40 antibody (R&D systems) in the presence of 100U/ml of recombinant human IL-4 (R&D systems).
Human cd40 antibody
Manufactured by R&D Systems
Sourced in United States
The Human CD40 Antibody is a monoclonal antibody that recognizes the CD40 protein expressed on the surface of human cells. CD40 is a member of the tumor necrosis factor receptor superfamily and plays a crucial role in the immune response. The antibody can be used for the detection and analysis of CD40 in various applications, such as flow cytometry, immunohistochemistry, and ELISA.
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Lab products found in correlation
2 protocols using human cd40 antibody
1
Isolation and Stimulation of B Cells
2
Isolation of Primary Myeloma Cells
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Blood samples collected from healthy volunteers and bone marrow (BM) aspirates from MM patients were processed by Ficoll-Paque (GE Healthcare Bio-Sciences, Pittsburgh, PA) gradient to obtain mononuclear cells. Normal B cells from healthy volunteers’ peripheral blood were enriched by negative selection methods using EasySep Human B Cell Isolation Kit (STEMCELL Technologies, Vancouver, Canada). For B cell proliferation, isolated B cells were stimulated by 10 μg/mL of human CD40 antibody (R&D systems, Minneapolis, MN, USA) in the presence of 100 U/mL of recombinant human IL4 (R&D Systems). Primary MM cells were further purified by CD138-positive selection using anti-CD138 magnetic-activated cell separation microbeads (Miltenyi Biotec, San Diego, CA). All procedures were performed using a protocol approved by the Institutional Review Board of the DFCI. Informed consent was obtained from all patients and healthy volunteers in accordance with the Declaration of Helsinki.
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