Flash 4.0v2

For the live imaging of transgenic Parhyale embryos, we utilized a custom-built multiview selective-plane illumination microscope^{26 (link)}. This microscope has two excitation and two detection branches. Both used water-dipping objectives (App LWD $5\times$ (numerical aperture, 1.1), Nikon Instruments, for detection and CFI Plan Fluor 10× (numerical aperture, 0.3) for excitation). Furthermore, each detection branch consisted of a filter wheel (HS-1032, Finger Lakes Instrumentation), with emission filters (BLP02-561R25, Semrock), tube lens ( $200\mathrm{m}\mathrm{m}$ , Nikon Instruments) and a camera (scientific complementary metaloxide–semiconductor; Hamamatsu Flash $4.0\mathrm{V}2$ ), with an effective pixel size of $0.262\mathrm{m}\mathrm{m}$ . The illumination branches featured a tube lens (200 mm, Nikon Instruments), scan lens (S4LFT0061/065, Sill Optics), galvanometric mirror $(\mathrm{6,215}\mathrm{h}$ , Cambridge Technology) and discrete laser line (561LS OBIS, $561\mathrm{n}\mathrm{m}$ ). The optical section employed a translation stage from Physik Instrumente (P-629.1CD with E-753 controller), rotation stage (U-628.03 with C-867 controller) and linear actuator (M-231.17 with C-863 controller).

For the live imaging of transgenic Parhyale embryos, we utilized a custom-built multiview selective-plane illumination microscope^{26 (link)}. This microscope has two excitation and two detection branches. Both used water-dipping objectives (App LWD $5\times$ (numerical aperture, 1.1), Nikon Instruments, for detection and CFI Plan Fluor 10× (numerical aperture, 0.3) for excitation). Furthermore, each detection branch consisted of a filter wheel (HS-1032, Finger Lakes Instrumentation), with emission filters (BLP02-561R25, Semrock), tube lens ( $200\mathrm{m}\mathrm{m}$ , Nikon Instruments) and a camera (scientific complementary metaloxide–semiconductor; Hamamatsu Flash $4.0\mathrm{V}2$ ), with an effective pixel size of $0.262\mathrm{m}\mathrm{m}$ . The illumination branches featured a tube lens (200 mm, Nikon Instruments), scan lens (S4LFT0061/065, Sill Optics), galvanometric mirror $(\mathrm{6,215}\mathrm{h}$ , Cambridge Technology) and discrete laser line (561LS OBIS, $561\mathrm{n}\mathrm{m}$ ). The optical section employed a translation stage from Physik Instrumente (P-629.1CD with E-753 controller), rotation stage (U-628.03 with C-867 controller) and linear actuator (M-231.17 with C-863 controller).