Genomic dna kit

We selected 9 different DNA samples to make the validation tests: NA12878, NA12891 and NA12892 as high quality DNA extracted from cell lines and with known genotypes and karyotypes; NT1 as control of high quality DNA extracted from a normal fallopian tube FFPE sample; T1, T2, T3, T4 and T5 as regular tumour FFPE samples with known somatic variants and CNVs. DNA was extracted using the QIAamp® DNA FFPE Tissue Kit (Qiagen, Ref: 56404) and DIN value for each of the 9 genomic samples was measured using a Tape Station 2200 (Agilent, Ref: G2965AA) and the Genomic DNA kit (Refs: 5067–5365 and 5067–5366).

In this study, we utilized C. graminicola strain M1.001. This strain was obtained from symptomatic maize plants during a survey of Colletotrichum spp. associated with anthracnose (Vaillancourt and Hanau, 1991 (link)) in Missouri in 1978. Total DNA was extracted from 3-days-old C. graminicola colonies grown in potato dextrose broth (PDB) under agitation (150 rpm) at 25°C (Sanz-Martín et al., 2016 (link)). The mycelium was vacuum filtered, immediately frozen in liquid nitrogen, and stored at −80°C until the DNA extraction.
The mycelium was macerated with liquid nitrogen using a mortar and pestle. One-third of the 1.5 mL Eppendorf tube was filled with powdered mycelium, and high molecular weight (HMW) DNA was extracted following a modified cetyltrimethylammonium bromide (CTAB) protocol for fungal genomic DNA (Murray and Thompson, 1980 (link); Baek and Kenerley, 1998 (link); Irfan et al., 2013 ). DNA was quantified by fluorometry (Qubit), and the DNA Integrity Number (DIN) was determined with one ScreenTape (5067-5365) of the Genomic DNA kit Agilent on the 2200 TapeStation system.