Western blot analysis was performed as detailed previously (26 (link)). Twenty micrograms of total cell proteins obtained from various cell lines were denatured and loaded on sodium dodecyl sulfate (SDS) polyacrylamide gels. The gels were electroblotted onto a polyvinylidene difluoride (PVDF) membrane for hybridization. The antibodies used for this investigation were from Abcam [TOM20 (ab56783), Total OXPHOS Human WB Antibody Cocktail (ab110411), Proteintech [β-actin (20536-1-AP), Twinkle (13435-1-AP), SSBP1 (12212-1-AP), ABclonal [PolγA (A1323), ELAC2 (A7128), TRNT1 (A17699), MRPP2 (A0959)], Invitrogen [POLRMT (PA5-28196)] and BOSTER [TFAM (BA2827)]. Peroxidase AffiniPure goat anti-mouse IgG and goat anti-rabbit IgG (Jackson) were used as secondary antibodies and protein signals were detected using the ECL system (CW- BIO). Quantification of density in each band was performed as detailed elsewhere (26 (link)).
Total oxphos human wb antibody cocktail
Manufactured by Proteintech
The Total OXPHOS Human WB Antibody Cocktail is a mixture of antibodies targeting various subunits of the oxidative phosphorylation (OXPHOS) complexes in human cells. This product can be used for the detection of OXPHOS complexes in western blot applications.
Automatically generated - may contain errors
2 protocols using total oxphos human wb antibody cocktail
1
Comprehensive Protein Expression Analysis
2
Western Blotting Analysis of Mitochondrial Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Western blotting analysis was carried out as detailed previously (23 (link), 42 (link)). 20 micrograms of total proteins obtained from lysed mitochondria were denatured and loaded on sodium dodecyl sulfate (SDS) polyacrylamide gels. The gels were electroblotted onto a polyvinylidene difluoride (PVDF) membrane for hybridization. The antibodies used for this investigation were from Abcam [ND1 (ab74257), ND3 (ab170681), ND6 (ab81212), TOM20 (ab56783), Total OXPHOS Human WB Antibody Cocktail (ab110411) and P62 (ab56416) ], Proteintech Group [CYTB (55090-1-AP), CO2 (55070-1-AP), ATP8 (26723-1-AP), CLPP (15698-A-AP), LONP1 (15440-1-AP), OPA1 (27733-1-AP), FIS1 (10956-1-AP), MFN1 (13798-1-AP), MFF (17090-1-AP), MFN2 (12186-1-AP) and DRP1 (12957-1-AP)], ABclonal Technology [β-Actin (AC026), ND4 (A9941), ND5 (A17972), CO1 (A17889), CO3 (A17891), LC3 (A19665), Parkin (A0968) and Cyt C (A4912)], Cell Signaling [Autophagy Antibody Sampler Kit (4445) and Apoptosis Antibody Sampler Kit (9915)] and Abcepta [PINK1 (AW5456)]. Peroxidase AffiniPure goat anti-mouse IgG and goat anti-rabbit IgG (Jackson) were used as secondary antibodies and protein signals were detected using the ECL system (CW-BIO). Quantification of density in each band was performed as detailed elsewhere (42 (link)).
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