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Xhoi and bglii

Manufactured by New England Biolabs
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XhoI and BglII are Type II restriction enzymes that cleave DNA at specific recognition sequences. XhoI recognizes and cleaves the palindromic DNA sequence 5'-C^TCGAG-3', while BglII recognizes and cleaves the palindromic DNA sequence 5'-A^GATCT-3'. These enzymes are commonly used in molecular biology applications such as DNA cloning, mapping, and analysis.

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Lab products found in correlation

Miniprep kit, by Qiagen (1 mentions) E coli jm109 cells, by Promega (1 mentions) Pgl4.23 luc2 minp vector, by Promega (1 mentions)

Close spelling variants of this product

BglII XhoI

2 protocols using xhoi and bglii

1

Cloning Luciferase Reporter Construct

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The PCR amplified fragments were digested using XhoI and BglII (New England Biolabs, MA, USA) and ligated into a pGL4.23[luc2/minP] vector (Promega E8411, Madison, MI, USA), previously digested using the same endonucleases. After an overnight incubation at 16°C, the ligation mixtures were transformed into competent E. coli JM109 cells (Promega). Plasmid DNA was isolated by Miniprep kit (Qiagen) according to the manufacturer’s instructions. All recombinant plasmid sequences were confirmed by Sanger sequencing.
Jiang K., Kessler H., Park Y., Sudman M., Thompson S.D, & Jarvis J.N. (2020). Broadening our understanding of the genetics of Juvenile Idiopathic Arthritis (JIA): Interrogation of three dimensional chromatin structures and genetic regulatory elements within JIA-associated risk loci. PLoS ONE, 15(7), e0235857.
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2

Cloning and Validating miR plasmids

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The pCMV miR144 plasmid was a generous gift from Dr. Eric Lai (Memorial Sloan Kettering Cancer Center). The insert for pCMV miR31 was generated by OE PCR using primers listed in Table S6. The insert was digested by XhoI and BglII (New England Biolabs) and ligated with digested pCMV vector. The ligated product was transformed into DH5α competent cells, and the plasmid integrity was verified by sequencing.
Ma S., Howden S.A, & Keane S.C. (2024). Use of steric blocking antisense oligonucleotides for the targeted inhibition of junction containing precursor microRNAs. bioRxiv.
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