We used a non-inverted industrial fluorescence microscope (Nikon LV150N, Tokyo, Japan) with brightfield, darkfield, simple polarizing, double beam interferometry, and DIC attachments. To visualize fluorescence images, a 20× objective lens (NA/WD = 0.45/4.5 mm) was used, and a DS-Ri2 microscope CMOS camera (Nikon, 6fps [4908 × 3246 pixels] to 45 fps [1636 × 1088 pixels]) was used to capture the fluorescence images. Fluorescence excitation was provided by a 100 W mercury lamp. The excitation and emission of propidium iodide (PI) dye and calcein-AM were EX 535–550 nm/DM 575 nm/DM 580 nm and EX 470–540 nm/DM 505 nm/EM 510 nm.
Ds ri2 microscope cmos camera
Manufactured by Nikon
The Nikon DS-Ri2 microscope CMOS camera is a high-resolution imaging device designed for use with Nikon microscopes. It features a 16.25-megapixel CMOS sensor and can capture images at a resolution of up to 4912 x 3264 pixels. The camera is capable of continuous shooting at up to 15 frames per second and supports a wide range of video formats. The DS-Ri2 is compatible with a variety of Nikon microscope models and is suitable for a range of laboratory applications.
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Lab products found in correlation
2 protocols using ds ri2 microscope cmos camera
1
Multimodal Fluorescence Microscopy Imaging
2
Multimodal Fluorescence Microscopy Imaging
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